| 锦灯笼药材木樨草苷含量测定 |
| |
| 引用本文： | 管慧洁,徐保利,李先宽,王冰. 锦灯笼药材木樨草苷含量测定[J]. 中华中医药学刊, 2012, 0(2): 296-298 |
| |
| 作者姓名： | 管慧洁 徐保利 李先宽 王冰 |
| |
| 作者单位： | 辽宁中医药大学药学院 |
| |
| 基金项目： | 国家科技部科技基础条件平台工作项目子课题(2005DKA21004) |
| |
| 摘 要： | 目的:对80个来源的锦灯笼药材木樨草苷的含量进行比较,为今后锦灯笼药材质量的系统评价及最适宜生长环境的研究奠定基础。方法:采用高效液相色谱法对锦灯笼木樨草苷含量测定,Agilent TC-18(4.6×150 mm,5μm)色谱柱,以乙腈-0.2%磷酸水为流动相,检测波长为350 nm,流速为1.0 mL.min-1。结果:木樨草苷在(0.148μg～0.74μg)成良好的线性关系,Y=32.778X+5.11,r=0.9998,加样回收率为100%,RSD=2.76%(n=5)。结论:不同产地的锦灯笼药材木樨草苷含量存在差异,并与生长环境存在一定关系。
|
| 关 键 词： | 锦灯笼 木樨草苷 高效液相色谱法 |
Determination of Luteoloside in Franchet Groumdcherry |
| |
| GUAN Hui-jie,XU Bao-li,LI Xian-kuan,WANG Bing. Determination of Luteoloside in Franchet Groumdcherry[J]. Chinese Archives of Traditional Chinese Medicine, 2012, 0(2): 296-298 |
| |
| Authors: | GUAN Hui-jie XU Bao-li LI Xian-kuan WANG Bing |
| |
| Affiliation: | (Liaoning University of Traditional Chinese Medicine,Dalian 116600,Liaoning,China) |
| |
| Abstract: | Objective:To determine luteoloside content in Franchet Groumdcherry which was collected from 80 locations.Method:Determination of the content of luteoloside used the method of HPLC.Luteoside was separated on a Agilent TC-18(4.6 150 mm,5 μm)column with acetonitrile-0.2% phosphoric acid(20∶ 80) as the mobile phase,with the flow rate of 1 mL·min-1,determined at 350 nm.Result:The liner range of luteoloside was 0.148 μg~0.74 μg(r=0.9998),Y=32.778X+ 5.11,r=0.9998,the average recovery rate was 100%,RSD =2.76%(n=5).Conclusion:There are significant differences in the content of luteoloside in Franchet Groumdcherry which was collected from different locations and there is a certain relationship between the growth environment and the content of the luteoloside. |
| |
| Keywords: | Franchet groumdcherry luteoloside HPLC |
| 本文献已被 CNKI 等数据库收录！ |
|
