铁皮石斛与市场上常见的几种伪品含糖量的测定

目的:分析比较铁皮石斛及常见伪品中多糖和甘露糖的含量。方法:采用高效液相色谱法测定石斛中甘露糖的含量,紫外-可见分光光度法测定多糖含量。结果:分析表明,各个不同的石斛药材多糖和甘露糖含量存在较大的差异。多糖含量最高的是齿瓣石斛,含量为44.43%;其次是报春石斛,含量为41.08%;含量最低的为细茎石斛,含量20.23%。甘露糖含量中,钩状石斛居首位,含量为34.17%;其次是齿瓣石斛,含量为32.96%;含量最低的为细茎石斛,含量为17.60%。结论:不同来源的铁皮石斛多糖和甘露糖含量均能达到2010年版《中国药典》要求,因此,传统以多糖和甘露糖为指标评价石斛质量是否合理有待进一步深入研究。

Determination of Sugar Content of Dendrobium Candidum and Several Common Counterfeit Drugs in the Market

Objective:To compare the content of polysaccharide and mannose in different kinds of dendrobium candium.Methods:Mannose in different types of dendrobium candium can be determined by HPLC and polysaccharide carried out by UV.Results:The analysis showed that the contents of polysaccharide and mannose in different kinds of dendrobium candium have obvious difference.The highest content of polysaccharide is the D.devonianum Paxt,which contains 44.43%;then the second one is the D.primulinum Lindl which contains 41.08%;the lowest is D.moniliforme,which only contains 20.23%.However,mannose content of D.aduncum Lindl.is highest,which contents 34.17%,the second is 32.96% in D.devonianum Paxt,the lowest for D.moniliformes is 17.60%.Conclusion:The content of polysaccharide and mannose in D.officinale Kimura et Migo from different sources can reach the 2010 edition of Chinese Pharmacopoeia requirement.Therefore,the conventional indicators of Polysaccharides and Mannose to evaluate the quality of Dendrobium is not reasonable.Further research should be developed.