天麻对病毒性心肌炎实验小鼠心肌细胞凋亡的影响

目的 研究天麻对病毒性心肌炎小鼠心肌细胞凋亡及caspase-3蛋白表达的影响,探讨天麻治疗病毒性心肌炎的作用及机制.方法 给Balb/c小鼠接种柯萨奇B3病毒制作病毒性心肌炎模型,感染病毒72 h后将仔活小鼠分为模型组、天麻组、氯沙坦组,并以未感染小鼠为正常对照组.大麻组、氯沙坦组分别予以相应药物干预7 d后,4组小鼠采用原位末端标记法(TUNEL)检测心肌细胞凋亡及Western blotting法检测caspase-3蛋白表达.结果 正常组未见到心肌细胞凋亡,模型组心肌细胞大量凋亡,凋亡率较正常组显著增加(P＜0.01),天麻组和氯沙坦组凋亡率较模型组显著降低(P＜0.05).模型组caspase-3蛋白表达较正常组显著增多(P＜0.01),天麻组和氯沙坦组较模型组显著降低(P＜0.05).结论 天麻能够抑制病毒性心肌炎心肌细胞的凋亡,通过caspase-3通路的调节,对心肌细胞起到保护作用.

The effects of Gastrodia elata on apoptosis in myocardial cells of mice with viral myocarditis

Objectives To investigate the effects of Gastrodia elata on apoptosis and expression of caspase-3 in myocardial cells of mice with Coxsackievirus B3(CVB3)infected viral myocarditis. Methods Forty-five Balb/c mice survived 72 h after CVB3 infection were randomly and equally divided into 3 groups,model group without being treated;Gastrodia elata group;Losartan group;another 15 uninfected Balb/c mice were chosen as control group. Seven days after being treated with Gastrodia elata or Losartan,myocardial apoptosis and the expression of caspase-3 in myocardial cells were detected using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay(TUNEL)and Western blotting in all groups. Results No apoptotic cells was detected in the control group;a great many of apoptotic cells were found in model group;The ratio of cardiocyte apoptosis was lower in both Gastrodia elata group and Losartan group than that in the model group(P < 0.05). The protein expression of caspase-3 in the model group were increased markedly in comparison with that of the control group(P < 0.01),and was also higher than that in Gastrodia elata group and Losartan group(both P < 0.05). Conclusions Gastrodia elata could inhibit apoptosis of cardiocytes through regulating the protein expression of caspase-3.