HPLC法测定利肝片中山柰素、槲皮素的含量

目的增加利肝片中山柰素、槲皮素HPLC法含量测定.方法采用高效液相色谱法;以十八烷基硅烷键合硅胶为填充剂;甲醇-0.4%磷酸溶液(50∶50)为流动相;检测波长360nm;流速1.0ml?min-1.结果山柰素在0.018～0.539μg,槲皮素在0.00538～0.1614μg范围内呈良好的线性关系(r=0.9999),平均加样回收率分别为101.1%、91.3%,RSD分别为1.4%、1.9%(n=5).结论本方法具有简便易行、重现性好,结果可靠、专属性强等特点,可用于该制剂的含量测定.

Determination of kaempferol and quercetin in Ligan Tablet by HPLC

OBJECTIVE To add the standard for the determination of kaempferide and quercetin in Ligan Tablet.METHODS HPLC method was selected to determine the contents of emodin.The C_ 18 column was used,the mobile phase was methanol-0.4% phoshoricacid solution(50∶50),detection wavelength was 360nm,flow rate was 1.0ml·min-1.RESULTS The calibration curve for Kaempferol was linear(r=0.9999)in the range of 0.018～0.539μg and the mean recovery was 101.1%,RSD was 1.4%(n=5);for quercetin the calibration curve was linear(r=0.9999)in the range of 0.00538～0.1614μg,and the mean recovery was 91.3%,RSD was 1.9%(n=5).CONCLUSION This method was accurate,simple,sensitive and reliable and it can be used for the determination of contents of Ligan Tablet.