苯乙酸对人结直肠癌细胞HCT-8的G1细胞周期阻滞及增殖抑制作用

摘要：目的 探讨诱导分化剂苯乙酸（PA）对人结直肠癌细胞系HCT 8细胞周期及增殖的影响。 方法 应用MTT比色法，以1.0，2.0，3.0，4.0，5.0 mmol/L的PA作用于体外培养的HCT 8细胞，分别于24，48，72h后对细胞增殖进行检测；流式细胞术分析细胞周期。结果 随着PA浓度的增加（1～5 mmol/L）或药物作用时间的延长（24～72h），肿瘤细胞生长抑制率明显增加。PA1~5mmol/L作用24h细胞生长抑制率为5.1%-24.3%，48h为16.7%-72.3%，72h为30.2%-93.4%。PA作用细胞72h后，G0/G1期比例显著下降，S期比例相对升高，组间差异显著（P＜0.05）。结论 PA在诱导分化结直肠癌HCT 8细胞过程中，可诱导G1细胞周期阻滞，抑制细胞增殖。

Phenylacetate inhibits cell proliferation via G1 cell cycle arrest in the HCT-8 colorectal carcinoma cell line

Objective To investigate the effect of phenylacetate(PA),a differentiation induce agent,on cell cycle and proliferation in colorectal carcinoma HCT-8 cell line.Methods HCT-8 cells were grown in the presence of PA(1.0,2.0,3.0,4.0,and 5.0mmol/L) for 24h,48h and 72h,respectively.The cellular proliferation inhibitory ratio was evaluated by MTT assay.The different phases of the cell cycle were analyzed using flow cytometer.Results HCT-8 cells were treated by 1.0-5.0 mmol/L PA for 24-72 h.With the increase of concentration of PA or the prolongation of the treatment time,the proliferation inhibitory ratio of the tumour cells increased notably by 5.1 %-24.3 % when treated for 24h,16.7 %-72.3 % when treated for 48 h,and 30.2 %-93.4 % when treated for 72 h,respectively.When HCT-8 cells were treated with 5.0 mmol/L PA for 72h,cell cycle analysis with flow cytometry showed that the percentage of HCT-8 cells in G0/G1 phase reduced significantly and S phase was relatively increased(P<0.05).Conclusions PA can arrest G1 cell cycle and inhibit cell proliferation.