p53蛋白与上颌窦鳞癌复发及预后的关系

目的 :探讨上颌窦鳞癌组织中 p5 3蛋白表达的诊断意义及其与复发及预后的关系。方法 :采用鼠单克隆抗体 DO- 7,免疫组化法检测 45例上颌窦鳞状细胞癌患者 (T2 N0 M0 )手术切除的癌组织中 p5 3蛋白含量 ,其过量表达提示 p5 3基因突变体存在。结果 :复发组中 88.2 %的癌组织中有 p5 3蛋白表达 ,显著高于对照组(2 8.6 % ) ,其差异有极显著性意义 (P <0 .0 1)。复发组中 6 1%的癌组织有较强的染色强度 ,高于对照组 (38% ) ,但其差异无显著性意义 (P >0 .0 5 )。结论 :p5 3蛋白可作为预测上颌窦鳞癌复发的基因水平的标记物 ,对其复发及预后具有重要意义。

The determination of p53 protein in maxillary sinus carcinoma and its prognosis value

OBJECTIVE: To determine whether p53 protein overexpression correlated with early tumor recurrence in carcinoma of maxillary sinus. METHOD: Forty-five patients with T2N0M0 squamous cell carcinoma of maxillary sinus were treated with operation and/or radiotherapy. The median follow up time was 3 years. The ratio of local control was 62.2% (28 patients), as control group; The ratio of local recurrence and/or metastasis was 37.8% (17 patients), as experiment group. Cancer specimens were obtained before treatment. p53 protein overexpression, which indicated a mutated p53 gene, was analyzed by immunohistochemistry, using the mouse monoclonal antibody DO-7. RESULT: Approximately 88.2% of the carcinoma that recurred locally overexpressed p53 protein, compared with only 28.6% of those with local control (P < 0.01). No significant relation was noted between p53 protein overexpression and pathohistologic grade. Intensity of staining did not predict tumor recurrence. CONCLUSION: The study demonstrated that the p53 protein is an important prognostic marker on gene level for maxillary sinus carcinoma.