抗辐灵对微波辐射致大鼠脑损伤的保护作用研究

目的探讨抗辐灵对微波辐射致大鼠脑损伤的保护作用。方法采用30 mW/cm2微波辐射100只雄性Wistar大鼠10 min,并于辐射前后7 d分别给予1 g×kg-1×d-1、2 g×kg-1×d-1和4 g×kg-1×d-1的抗辐灵,每日1次,连续14 d。采用Morris水迷宫检测大鼠学习和记忆能力,HPLC检测大鼠海马组织中氨基酸类神经递质含量,光镜及电镜观察海马组织学和超微结构,并采用图像分析方法对海马组织中固缩神经元的数密度、突触间隙宽度、突触后致密物厚度和致密物长度进行定量检测。结果 30 mW/cm2微波辐射后7 d大鼠平均逃避潜伏期明显升高;停药后1、2、3 d,各给药组的平均逃避潜伏期明显低于辐射对照组;30 mW/cm2微波辐射后7 d,甘氨酸（Gly）和γ-氨基丁酸（GABA）含量下降,而2 g×kg-1×d-1和4 g×kg-1×d-1给药组Gly、GABA含量明显上升;30 mW/cm2微波辐射后7 d大鼠海马组织结构和超微结构损伤,表现为固缩神经元增多,线粒体肿胀空化,突触间隙不清、突触后致密物增厚。2 g×kg-1×d-1和4 g×kg-1×d-1给药组损伤明显较辐射组轻。结论 30 mW/cm2微波辐射可损伤大鼠学习记忆能力和脑组织结构,造成氨基酸类神经递质代谢紊乱;给予2 g×kg-1×d-1、4 g×kg-1×d-1抗辐灵对微波辐射所致的上述损伤具有保护作用。

Protective effects of Kang Fu Ling on brain injury induced by microwave radiation in rats

Objective To evaluate the effects of Kang Fu Ling（KFL）, a compound Chinese traditional medicine,against brain injury induced by microwave radiation. Methods Male Wistar rats were irradiated with 30 mW/cm2 microwave for 10 minutes and orally administrated with KFL（0,1,2,4 g × kg-1× d-1） 7 d before and after radiation, once/d, successive administration for 14 d. The learning and memory abil- ities of rats were detected by Morris water maze. Amino acid neurotransmitters in hippocampus were tested by HPLC. The morphological changes of hippocampus tissues were observed by light and electron micros- copy. The numerical density of pyknotic neurons, width of synaptic cleft, thickness and length of postsyn- aptic density were measured by image analysis. Result 30 mW/cm2 microwave radiation impaired rats learning and memory 7 d after microwave exposure. And KFL treatment improved their performance in be- havior test. The contents of glycine changes were reversed in 2 g × kg-1 ×d-1 and gamma aminobutyric acid were decreased after radiation. group and 4 g × kg-1 ×d - 1 group. The structure of hip pus was damaged by microwave radiation. Neurons exhibited pyknosis and anachromasis, while th These ynaptic density was increased. The injury of KFL treatment groups was lighter than that of microwave ex- posed rats. Conclusion 30 mW/cm2 microwave bolic disturbance of amino acids neurotransmitters radiation may induce the injury of structure and metain hippocampus, and impair the learning and memory of ：rats. KFL has protective effects against brain injury caused by microwave radiation with a certain extent.