马鞭草提取液C部位对人绒毛膜癌JAR细胞增殖的影响

目的：探讨马鞭草(Verbena officinalis)提取液C部位对人绒毛膜癌JAR细胞增殖的影响。方法：通过相差显微镜观察活细胞贴壁程度、细胞形态改变；MTT法测定不同浓度C部位在不同时段对JAR细胞的增殖抑制率，结果用方差分析进行统计学检验：流式细胞仪检测细胞阻滞周期。结果：马鞭草C部位可引起细胞数目减少并萎缩；细胞增殖抑制率随着时间的延长和浓度的增加逐渐增大．72h的40mg／ml剂量组抑制最明显，抑制率为65％；流式细胞仪检测结果显示阻滞周期在GJM期之间。结论：马鞭草提取液C部位对人绒毛膜癌JAR细胞增殖有明显抑制作用，其作用机制有待进一步探索。

Inhibitory Effects of the Part C in Alcohol Extract of Verbena Officinalis on Human Choriocarcinoma JAR Cell Line

Objective: To investigate the effect of the Part C in Alcohol Extract of Verbena officinalis on Human Choriocarcinoma JAR Cell Line. Methods: Firstly, morphological changes of JAR cells were observed with phase-contrst microscope. Secondly, JAR cells were determined in the proliferation-inhibition ratio of the part C with vary chroma during different period of time using MTT method.And thirdly, flow cytometry(FCM) was performed for measurement of phase of cell cycle. Results: The incubated tumor cells were decreased in number and became atrophic when they were treated with the part C. The inhibition rate of experimental groups was more than that in control group and increased according to increasement of time and concentration. In 72 h?40 mg/ml, there was a significant control and its inhibition was 65%. Phase of cell cycle analysis by FCM showed that cells were arrested in G2/M phase. Conclusion: The part C in Alcohol Extract of Verbena officinalis is a highly effective antineoplastic agent to choriocarcinoma cells, manifestating time-concentration effect.