| HPLC法测定清肺饮中绿原酸的含量 |
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| 引用本文： | 王跃茹,王利,陈卫东. HPLC法测定清肺饮中绿原酸的含量[J]. 安徽医药, 2009, 13(2): 156-157 |
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| 作者姓名： | 王跃茹 王利 陈卫东 |
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| 作者单位： | 安徽省马鞍山市第十七冶医院,安徽,马鞍山,243000;安徽省马鞍山市第十七冶医院,安徽,马鞍山,243000;安徽省马鞍山市第十七冶医院,安徽,马鞍山,243000 |
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| 摘 要： | 目的建立清肺饮的质量控制方法。用HPLC法测定清肺饮中绿原酸的含量。方法采用Sinochorom ODS C18柱，以乙腈-0．4％磷酸水溶液（13：87）为流动相；流速为1．0ml·min^-1；检测波长为327nm。结果绿原酸在27．76～138．8mg·L^-1。范围内线性关系良好，r=0．9999；平均加样回收率为98．75％，RSD为1．1％（n=5）。结论本法专属性强，准确度高，重现性好，可作为该产品质量控制的方法。
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| 关 键 词： | 高效液相色谱法 清肺饮 绿原酸 |
Determination of chlorogenic acid in qingfeiyin by HPLC |
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| WANG Yue-ru,WANG Li,CHEN Wei-dong. Determination of chlorogenic acid in qingfeiyin by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2009, 13(2): 156-157 |
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| Authors: | WANG Yue-ru WANG Li CHEN Wei-dong |
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| Affiliation: | WANG Yue-ru,WANG Li,CHEN Wei-dong(Maanshan Hospital of 17th Metallurgical Construction Company,Maanshan 243000) |
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| Abstract: | Aim To establish a method for determination of chlorogenic acid in qingfeiyin by HPLC. Methods The separation was performed on SinochoromODS C18 column,with acetonitrile-0.4% phosphoric acid （13：87 ）as mobile phase. The detection wavelength was 327 nm. The flow rate was 1.0 ml·min^-1. Results The linear range of chlorogenic acid was from 27.76 to 138.8 mg · L^-1 （ r = 0. 9 999）. The average recovery was 98.75%, and RSD was 1.1% （ n = 5 ）. Conclusion The method is simple and accurate. It can be used for the quality control of qingfeiyin. |
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| Keywords: | HPLC qingfeiyin chlorogenic acid |
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