水通道蛋白1在喉癌组织中的表达及分布

目的：明确水通道蛋白1（AQP1）在喉癌组织中的表达和分布，并探讨其在喉癌发病中可能的作用机制及意义。方法：取喉癌组织20例及癌旁正常组织15例，应用RT-PCR，Western blot，免疫组织化学技术检测AQP1蛋白在喉癌和正常对照组织中的表达及分布。结果：AQP1表达于正常喉黏膜固有层的血管内皮细胞及喉黏膜腺体上。在喉癌组中主要表达于肿瘤的血管内皮细胞，在肿瘤上皮细胞和癌巢中亦有表达。喉癌中AQP1 mRNA及蛋白表达水平较癌旁正常组织增多，二者之间差异有统计学意义。结论：AQP1在喉癌组织中表达增高，在癌旁正常组织中较低，提示AQP1在喉癌发病中的重要作用有待深入研究。

Expression and distribution of aquaporin 1 in laryngeal carcinoma

Objective:To detect expression level and distribution of aquaporin 1(AQP1)in laryngeal carcinoma tissues and try to find out the significance about the mechanism of AQP1 in the invasion of laryngeal carcinoma.Method:Twenty cases of laryngeal carcinoma tissues and 15 cases of normal tissues beside tumors were acquired immediately after surgery.The expression and distribution of aquaporin 1 in laryngeal carcinoma were examined by RT-PCR,westernblot analysis and immunohistochemical technique.Result:AQP1 expresses on the capillary endothelia of laryngeal mucous lamina propria and laryngeal mucous glands of normal tissues and mainly on the capillary endothelia cells,tumor cells and cancer nests of laryngeal carcinoma tissues.AQP1 mRNA and protein expression level in laryngeal tumor tissues is remarkably stronger than that in normal tissues beside tumors.Conclusion:The expression of AQP1 was increased in laryngeal carcinomar tissues over normal laryngeal tissues.It suggests that the mechanism of AQP1 in laryngeal carcinoma etiopathogenisis need to be studied.