热化疗联合维拉帕米对人乳腺癌耐药细胞化疗敏感性的实验研究

目的观察用热化疗与维拉帕米合用是否能抑制人乳腺癌耐药细胞株MCF-7/ADM多药耐药蛋白P-gp的表达以逆转乳腺癌对化疗药的耐药性,提高化疗敏感性。方法高表达P-gp的MCF-7/ADM,在37~43℃热化疗条件下分别加入3μg/mL阿霉素(ADM)和不同浓度的逆转剂维拉帕米(VRP,0~5μmol/L),用共聚焦MRC-1024(60×物镜)观察扫描,Laserpix软件检测细胞内ADM的荧光强度,流式细胞仪检测凋亡。结果随着温度和VRP浓度的升高,MCF-7/ADM细胞内ADM荧光强度升高,凋亡率增加,且温度和浓度有协同作用。结论热化疗与维拉帕米联合可有效逆转P-gp所致耐药,提高化疗敏感性。

Experimental Study on Thermochemotherapy Combined with Verapamil for Reversing the Multidrug Resistance Responsible to Breast Cancer Cell

OBJECTIVE: To reverse the multidrug resistance of MCF-7/ADM cell line by thermochemotherapy combined with verapamil (VRP). METHODS: The drug resistance cells MCF-7/ADM were treated with 3 microg/mL Adriamycin and different concentration VRP (0-5 micromol/L), and then cultured with 37-43 degrees C for 30 minutes. Confocal microscopy was used to demonstrate the fluorescence adriamycin in cells, and the cell apoptosis rate was analyzed with flow cytometry. RESULTS: With the increases of thermochemical temperature and VRP concentration, the cell adriamycin fluorescence intensity and apoptosis rate of MCF-7/ADM were raised. Thermochemotherapy showed marked synergistic effect with VRP in MCF-7/ADM. CONCLUSION: It is suggested that thermochemotherapy combined with Verapamil could be used as a way to overcome the multidrug resistance.