健脾疏肝降脂方干预非酒精性脂肪肝病肥胖小鼠肝功能、游离脂肪酸机制探讨

目的:观察健脾疏肝降脂方治疗非酒精性脂肪肝(NAFLD)肥胖小鼠ALT、AST、FFA及光镜下病理组织学改变,并与壳脂胶囊治疗组比较,观察其疗效。方法:将72只小鼠随机分为模型组、健脾疏肝降脂方高、中、低剂量组、壳脂胶囊对照组和空白组。采用饲养高脂饲料及皮下注射5%CCl₄复制NAFLD肥胖小鼠模型,分别以相应药物灌胃6周,检测血清中ALT、AST及FFA的含量,光镜下观察肝组织的脂肪变程度。结果:①各个用药组相比模型组,血清ALT、AST均降低有统计学意义(P<0.01),且中药各浓度组较壳脂胶囊组血清ALT、AST降低,差异有统计学意义(P<0.05); 中药高、中剂量组相比模型组FFA降低,比较有统计学意义(P<0.05); 但与壳脂胶囊组相比,中药各剂量组改善FFA,差异无明显统计学意义(P>0.05)。②光镜可见:各用药组光镜下病理组织改变均较模型组明显,以中、高剂量组较低剂量组小鼠肝细胞脂肪变程度减轻较明显。结论:健脾疏肝降脂方能有效降低NAFLD肥胖小鼠的肝功能、其与浓度无关; 可以降低FFA及改善光镜下肝脏的脂肪变,其有效程度与浓度有一定的关系。

Effect and mechanism of Jianpi Shugan Jiangzhi recipe on liver function and FFA in NAFLD obese mice

Objective:To observe the histopathological changes of ALT,AST,FFA and light microscope in the treatment of NAFLD obese mice by Jianpi Shugan Jiangzhi Recipe.And compared with the treatment group of shell fat capsule to observe the curative effect.Methods:Seventy-two mice were randomly divided into the model group,the high-dose,medium-dose and low-dose groups of Jianpi Shugan Jiangzhi Recipe,the shell fat capsule control group and the blank group.High-fat diet and subcutaneous injection of 5%CCL-4 were used to replicate NAFLD in obese mice,and corresponding drugs were given to the mice for 6 weeks,The contents of ALT、AST and FFA in serum were detected,The degree of fatty change in liver tissue was observed under light microscope.Results:①Compared with the model group, the serum ALT and AST levels of each treatment group were significantly lower(P<0.01).In addition,the serum ALT and AST levels in each concentration group of traditional Chinese medicine were significantly lower than those in the shell fat capsule group(P<0.05); Compared with the model group, FFA in the high-dose and medium-dose groups of traditional Chinese medicine decreased significantly(P<0.05).However,compared with the shell fat capsule group,the improvement of FFA in each dose group of traditional Chinese medicine was not statistically significant(P>0.05).②The histopathological changes were more obvious in each group under light microscope than in the model group,Among them,the medium and high dose group and low dose group mice hepatocyte fatty change extent reduce obviously.Conclusion:Jianpi Shugan Jiangzhi Recipe can effectively reduce liver function in obese NAFLD rats,it has nothing to do with the concentration; It can reduce FFA and improve hepatic adiposis under light microscope,The effective degree is related to the concentration.