白细胞介素17在大鼠慢性阻塞性肺疾病和支气管哮喘模型中的变化及意义

目的了解白细胞介素17(IL17)在大鼠慢性阻塞性肺疾病(COPD)和支气管哮喘(简称哮喘)模型中的变化。方法SD雄性大鼠随机分为COPD组(13只)、哮喘组(11只)、吸烟组(11只)和正常对照组(12只);观察各组大鼠气道病理改变,采用酶联免疫吸附测定(ELISA)法检测大鼠支气管肺泡灌洗液(BALF)及肺组织匀浆IL17水平;免疫组化及图像分析法检测肺组织IL17及胞间黏附分子1(ICAM1),CD3、CD4、CD8的表达。结果COPD组和哮喘组肺组织匀浆IL17和BALF中IL17均显著增高,与正常对照组和吸烟组比较差异有显著性(P均<001);COPD组IL17主要在支气管上皮细胞内表达,哮喘组则在气道周围单核淋巴细胞内表达;COPD组和哮喘组IL17表达量均显著高于正常对照组及吸烟组(P<001或<005)。COPD组肺组织匀浆IL17与BALF中中性粒细胞(r=0676,P=0002)以及BALF中IL17与小气道平滑肌增生均呈正相关(r=0641,P=0046);肺组织匀浆IL17与ICAM1呈正相关(r=0550,P=0051);哮喘组肺组织匀浆IL17分别与BALF中嗜酸粒细胞(r=0884,P=0001)和肺组织CD3T细胞呈正相关(r=0812,P=0002)。结论COPD中IL17可能通过增强ICAM1的表达等途径促进中性粒细胞在气道内聚集,参与对COPD气道炎症的调节。IL17在哮喘中的作用可能与其促进嗜酸粒细胞在气道内聚集有关。

The changes and significance of interleukin-17 in rat models of chronic obstructive pulmonary disease and asthma

Objective To investigate the changes of interleukin 17(IL 17) both in rat models of chronic obstructive pulmonary disease(COPD) and in asthma Methods Male SD rats were randomly divided into COPD group,asthma group,smoking group and control group The concentrations of IL 17 in lung tissues and in bronchoalveolar lavage fluid (BALF) were measured by enzyme linked immunosorbent assay(ELISA) The expression of IL 17 in lung tissue was determined by immunohistochemical staining and photographic analysis Results The concentrations of IL 17 both in the lung tissue and in the BALF were significantly higher in the COPD group and the asthma group than in the controls and the smoking group(all P <0 01) IL 17 was mainly expressed in airway epithelial cells of COPD rats and in T lymphocytes around the airway in rats with asthma The levels of IL 17 were also higher both in the COPD group and in the asthma group than in the smoking group and the controls(all P <0 01) evaluated by immunohistochemical staining and photorgraphic analysis In the COPD group,IL 17 concentration of lung tissue was positively correlated with percentage of neutrophils in BALF; it also tended to be positively correlated with the level of intercellular adhesion molecule 1(ICAM 1) in airway epithelial cells,and the concentration of IL 17 in BALF was positively correlated with the degree of smooth muscle proliferation as well The concentration of IL 17 in the lung tissue of the asthma group was positively correlated with the number of eosinophils in BALF and with the number of CD 3 T lymphocytes in lung tissues respectively Conclusions The production of IL 17 in COPD and in asthma might be distinct IL 17 probably recruits neutrophils into airways by enhancing the expression of ICAM 1 in airway epithelial cells,and it might play a role in pathological changes of small airways in COPD The role of IL 17 in asthma maybe associated with the recruitment of eosinophils into airways