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| 丙型肝炎病毒非结构蛋白5A调节新生多肽复合物启动子转录活性的研究 | |
| 引用本文： | 戴久增,成军,曲建慧,纪冬. 丙型肝炎病毒非结构蛋白5A调节新生多肽复合物启动子转录活性的研究[J]. 中华肝脏病杂志, 2005, 13(8): 579-581 |
| 作者姓名： | 戴久增 成军 曲建慧 纪冬 |
| 作者单位： | 100039,北京,解放军第三0二医院传染病研究所基因治疗研究中心 |
| 摘 要： | 目的探讨丙型肝炎病毒（HCV）非结构蛋白5A（NS5A）对新生多肽相关复合物（NACA）启动子转录活性的影响。方法以我室构建的能够表达NS5A蛋白的pcDNA3．1（-）-NS5A质粒和含有NACA基因启动子的PCAT3-NACA报告基因质粒共转染HepG2细胞系设为实验组，同时PCAT3-NACA单独转染HepG2细胞系作为对照组。用酶联免疫吸附法检测氯霉素乙酰转移酶（CAT）的表达活性。结果pCAT3-NACA单独转染HepG2细胞的CAT酶表达活性与pCAT3-NACA和pcDNA3．1（-）-NS5A共转染组HepG2细胞的CAT酶表达活性比较，共转染组A值下降了79．3％。结论HCV NS5A能够抑制NACA启动子的活性，对NACA的表达具有下调作用。 |
| 关 键 词： | 丙型肝炎病毒 非结构蛋白5A 调节作用 多肽复合物 启动子 转录活性 NACA HCV |
| 收稿时间： | 2004-09-10 |
| 修稿时间： | 2004-09-10 |
Study of the down-regulating effect of hepatitis C virus NS5A protein on NACA promoter |
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| DAI Jiu-zeng,CHENG Jun,QU Jian-hui,Ji Dong. Study of the down-regulating effect of hepatitis C virus NS5A protein on NACA promoter[J]. Chinese journal of hepatology, 2005, 13(8): 579-581 | |
| Authors: | DAI Jiu-zeng CHENG Jun QU Jian-hui Ji Dong |
| Affiliation: | Gene Therapy Research Center, Institute of Infectious Diseases, 302 Hospital of PLA, Beijing 100039, China. daijiuzeng18@163.com |
| Abstract: | Objective To investigate the effect of hepatitis C virus (HCV) non-structure protein NS5A on the activity of calcium-regulating protein alpha subunit of nascent polypeptide-associated complex (NACA) promoter. Methods HepG2 cell plasmid pCAT3-NACA, containing NACA promoter, was transfected alone or cotransfected with pcDNA3.1(-)-NS5A, and chloramphenicol acetyl transferase (CAT) enzyme activity was assayed by enzyme-linked immunoassay (ELISA). Results The CAT activity in the pcDNA3.1(-)-NS5A cotransfection group was 20.7% of the CAT activity in the pCAT3-NACA group. Conclusion HCV non-structural protein NS5A has a down-regulating effect on the promoter of NACA gene. |
| Keywords: | Hepacivirus Viral nonstructural proteins NACA |
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