参灵方防治佐剂性关节炎大鼠药源性胃黏膜损伤的实验研究

目的探讨参灵方对佐剂性关节炎(adjuvant arthritis,AA)大鼠药源性胃黏膜损伤的防治作用。方法建立大鼠AA模型,造模后将出现二次反应的大鼠随机取48只分为模型组、复合模型组、雷尼替丁组、参灵方小剂量组、参灵方中剂量组、参灵方大剂量组,加上空白对照组,每组8只,共计7组。雷尼替丁组予雷尼替丁30 mg/(kg.d)灌胃,参灵方小剂量组、中剂量组、大剂量组分别予参灵方3.5 g、7.0 g、14.0 g/(kg.d)灌胃,空白对照组、模型组和复合模型组分别予等容积0.9%氯化钠注射液灌胃,均为14 d。计算治疗前后大鼠足爪肿胀率、关节炎指数及胃黏膜损伤指数、各灌药组对胃黏膜损伤的抑制率、胃黏膜的病理学变化,并进行组间比较。结果给药前后模型组、复合模型组、雷尼替丁组、参灵方各剂量组组间关节炎指数、足爪肿胀率差异均无统计学意义(P>0.05);给药前后各组与空白对照组比较,足爪肿胀率差异均有统计学意义(P<0.05);参灵方治疗后大鼠足爪容积、关节炎指数较模型组、复合模型组均有所下降,但差异无统计学意义(P>0.05)。雷尼替丁组、参灵方小剂量、中剂量、大剂量组胃黏膜损伤指数与复合模型组比较差异均有统计学意义(P<0.01),参灵方小剂量、中剂量组与雷尼替丁组比较胃黏膜损伤指数差异无统计学意义(P>0.05),而参灵方大剂量组与雷尼替丁组比较差异有统计学意义(P<0.05)。雷尼替丁组、参灵方小剂量、中剂量、大剂量组胃黏膜损伤抑制率分别为74.05%、66.81%、74.41%、55.70%,参灵方小剂量组、大剂量组与中剂量组及雷尼替丁组比较差异有统计学意义(P<0.05)。结论参灵方对AA大鼠关节无明显影响,但对药源性胃黏膜损伤有保护作用。

An Experimental Study of Shenling Decoction in Prevention of Drug-Induced Gastric Mucosa Injury in Adjuvant Arthritis Rats

Objective To investigate the effect of Shenling decoction against drug-induced gastric mucosa injury in adjuvant arthritis(AA) rats.Methods The complex rat models of AA and drug damage were rendered.After 2 times of reaction,48 rat models were randomly selected and divided into 7 groups: model group,compound model group,Ranitidine group,Shenling decoction low-dose group,Shenling decoction middle-dose group,Shenling decoction high-dose group,and blank control group(8 each).Ranitidine group underwent intragas...