槐定碱对小鼠腹腔巨噬细胞功能的影响

观察槐定碱对巨噬细胞功能的影响。以 YC花环试验、活化 MΦ的 NO诱生量及胞内溶菌酶与超氧化物岐化酶的活性、小鼠脾脏和胸腺的组织学形态变化为观察指标。结果 ,灌胃槐定碱小鼠的腹腔 MΦC3 b受体数量较对照组显著升高 ;与对照组比较 ,脾组织中脾小体融合增大 ,MΦ增多。槐定碱不影响腹腔活化 MΦ内 SOD活性 ,但对腹腔活化 MΦ的 NO诱生量及胞内 L SZ活性有抑制趋势。结论 ,槐定碱可增强正常小鼠 MΦ功能 ,并促进脾脏免疫细胞增殖 ,而对活化 MΦ的功能则表现抑制倾向。

Effects of Sophoridine on the Function of Peritoneal Macrophages in Mice

Objective:To observe the effects of Sophoridine (SR) on the function of macrophages (MΦ).Methods:YC-rosettes forming test,nitric oxide(NO) production of activated MΦ,activities of lysozyme(LSZ) and superoxide dismutase(SOD) in activated MΦ,histologic changes of spleen and thymus of mice were used as the parameters of observation.Results:The number of C 3b receptors on the peritoneal macrophages from SR-treated mice were higher than that of the control.Compared with the control,the malpighian corpuscles of spleen were enlarged and the number of MΦ were increased in the splenic tissue.SR didn't affect the SOD activity,but there was the inhibitory trend on the NO production and the LSZ activity of the peritoneal MΦ activated by thioglycollate(TG)(no statistical significance).Conclusion:SR could enhance the function of MΦ in normal mice and promote the proliferation of immunologic cells in the spleen,but there was the inhibitory trend on the function of activated MΦ.