| 热休克蛋白70与胎盘血管病中内皮激活的相关研究 |
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| 引用本文： | 刘彦霞,徐健,王欣. 热休克蛋白70与胎盘血管病中内皮激活的相关研究[J]. 实用妇产科杂志, 2010, 26(3) |
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| 作者姓名： | 刘彦霞 徐健 王欣 |
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| 作者单位： | 山东大学附属省立医院,山东,济南,250021 |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 目的:进一步阐明胎盘血管病(PVD)的发病机制.方法:选取胎盘血管病组(研究组)与正常孕妇(对照组)两组标本,选择应用寡核苷酸芯片技术筛选出差异表达水平较高的基因,定量PCR和Western-blot验证表达,并应用免疫组化检测定位.结果:与对照组相比,Hsp A1A和HspA6表达水平显著改变,且共同编码热休克蛋白(Hsp 70);Hsp 70在胎盘微血管中主要表达在内皮细胞和平滑肌细胞;研究组与对照组相比,Hsp 70 mRNA和蛋白的表达均增高(P<0.05);Hsp 70 mRNA和蛋白的表达均与胎儿出生时的体重呈负相关(P<0.05).结论:Hsp 70在PVD的发病机制中是极其重要的,Hsp 70高表达可能引起了内在的免疫应答和炎性反应.
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| 关 键 词： | 基因芯片 热休克蛋白70 内皮激活 胎盘血管病 |
Study of Relationship between Hsp70 and Endothelial Activation inPlacental Vascular Diseases |
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| LIU Yanxia,XU Jian,WANG Xin. Study of Relationship between Hsp70 and Endothelial Activation inPlacental Vascular Diseases[J]. Journal of Practical Obstetrics and Gynecology, 2010, 26(3) |
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| Authors: | LIU Yanxia XU Jian WANG Xin |
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| Affiliation: | LIU Yanxia,XU Jian,WANG Xin(Provincial Hospital affiliated to Sh,ong University,Jinan Sh,ong 250021,China)Corresponding author:WANG Xin |
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| Abstract: | Objective:The pathogenesis of placental vascular disease(PVD) was further investigated.Methods:PVD group (study group) and normal pregnancy (control group) were recruited. Two-channel microarray technique was applied to screen the most differently expressive genes of human genome,and verified the different expressions in mRNA and protein levels by quantitative PCR and Western-blot, also localized the expression of different genes in placental tissue by immunohistochemistry.Results:HspA6 and HspA1A had extre... |
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| Keywords: | Gene arrays Heat shock protein70 Endothelial activation Placental vascular disease |
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