复方六月青对肝纤维化大鼠肝脏Ⅰ型胶原合成的抑制作用

目的:研究复方六月青(CLYQ)对四氯化碳(CCl4)致肝纤维化大鼠肝组织Ⅰ型胶原(Col I)合成的抑制作用。方法:SD大鼠sc CCl4建立肝纤维化模型,随机分为5组:模型对照组、秋水仙碱组、CLYQ高剂量组、中剂量组、低剂量组,并设正常SD大鼠为空白对照组。模型组和空白对照组ig生理盐水,其他组ig相应的药物,连续4周。采用实时荧光定量PCR法检测各组肝组织Col I mRNA表达情况,采用免疫组织化学法检测各组肝组织Col I蛋白表达情况。结果:与模型对照组比,CLYQ各剂量组大鼠肝组织Col I mRNA的表达均下调(P0.01),且表现一定的剂量依赖性;CLYQ各剂量组大鼠肝组织Col I蛋白阳性表达面积和强度显著减少和减弱(P0.01或P0.05),且呈明显的剂量依赖性。结论:CLYQ可下调肝纤维化大鼠肝组织Col I mRNA表达及其蛋白质合成以抑制细胞外基质的沉积,从而发挥其抗肝纤维化的作用。

Inhibitory Effect of Compound Liuyueqing on Synthesis of Collagen Type I in Hepatic Tissues in Rats with Hepatic Fibrosis

ObJective: To study the inhibitory effect of Compound Liuyueqing(CLYQ) on the synthesis of Collagen type I (Col I) in hepatic tissues in rats with hepatic fibrosis. Method: Hepatic fibrosis model of SD rats was induced by subcutaneous inJection (sc) of CCl₄. Hepatic fibrosis SD rats were divided into 5 groups randomly: model control group, colchicina-group, high dose, middle dose and low dose of CLYQ groups, and normal SD rats were the blank contol group. Model control group and blank contol group were treated with normal saline, and the other groups were treated with corresponding drugs for four consecutive weeks by intragastric administration (ig). The relative quantification of Col I mRNA expression in hepatic tissues was detected by real time fluorescence quantitative PCR, and the quantification of Col I protein expression was detected by immunohistochemistry. Result: Compared with the model group, the various dosage groups of CLYQ could down regulate significantly Col I mRNA expression (P<0.01) and showed a dose-dependent relationship according to the results of real time fluorescence quantitative PCR. The results of immunohistochemistry showed that after treamented with CLYQ the area and the intensity of Col I protein expression in hepatic tissues were reduced and weakened significantly (P<0.05, or P<0.01) compared with that of the model group. Col I protein expression was reduced obviously in a dose=dependent manner. Conclusion: CLYQ can down-regulate the expression of Col I mRNA and protein synthesis in hepatic tissues of hepatic fibrosis rats. The results suggest that CLYQ can reduce the collagen, thus suppressing the deposition of ECM. Hence CLYQ has the marked effect of anti-hepatic fibrosis.