HPLC法同时测定复方儿茶酊中儿茶素与原儿茶素的含量

目的：建立同时测定复方儿茶酊中儿茶素与原儿茶素含量的方法。方法：采用高效液相色谱法。色谱柱为YMC ODS（250mm×4.6mm,5μm）,流动相为0.04mol·L-1枸橼酸溶液-N,N-二甲基甲酰胺-四氢呋喃（45∶8∶2,V/V/V）,流速为1.0mL·min-1,检测波长为280nm,柱温为35℃。结果：儿茶素与原儿茶素的检测浓度分别在0.0518～0.2590、0.0101～0.2030mg·mL-1（r均为0.9999）范围内与各自峰面积积分值呈良好的线性关系;平均加样回收率分别为101.63%和100.49%,RSD分别为1.27%和1.12%（n均为9）。结论：本方法简便、准确、稳定,能有效控制复方儿茶酊的质量。

Content Determination of Catechin and Epicatechin in Compound Catechu Tincture by HPLC

OBJECTIVE：To establish the method for the content determination of catechin and epicatechin in Compound catechu tincture.METHODS：HPLC method was adopted.The determination was performed on YMC ODS（250 mm×4.6 mm,5 μm） column with mobile phase consisted of 0.04 mol·L-1 citric acid solution-N,N-dimethylformamide-tetrahydrofuran（45：8：2,V/V/V） at the flow rate of 1.0 mL·min-1.The detection wavelength was 280 nm and column temperature was 35 ℃.RESULTS：The linear range of catechin and epicatechin were 0.051 8～0.259 0 mg·mL-1（r=0.999 9） and 0.010 1～0.203 0 mg·mL-1（r=0.999 9）.The average recoveries were 101.63%（RSD=1.27%,n=9） and 100.49%（RSD=1.12%,n=9）.CONCLUSION：The method is simple,accurate and stable for quality control of Compound catechu tincture.