中国HCV/HIV合并感染者淋巴细胞CD25表达和血清IP-10的研究

目的研究淋巴细胞表面受体CD25和血清干扰素诱导蛋白10（IP-10）,在丙型肝炎病毒（HCV）单纯感染,人类免疫缺陷病毒（HIV）单纯感染和HCV/HIV合并感染中的表达及意义。方法采用流式细胞术,检测HCV感染组（n=21）、HIV感染组（n=14）、HCV/HIV感染组（n=28）及正常对照组（n=30）,T淋巴细胞表面CD25的表达,ELISA方法检测血清IP-10含量。结果血清IP-10水平在HCV感染组、HIV感染组和HCV/HIV合并感染组都明显升高,HIV感染组及HCV/HIV合并感染组CD4＋T细胞表面CD25表达显著降低（P〈0.01）;HCV感染组CD4＋T细胞表面CD25表达轻度升高,但差异无统计学意义。结论中国HCV/HIV合并感染与CD4＋T细胞CD25表达及血清IP-10水平密切相关。

Surface Expression of CD25 from PBMC and Levels of Serum IP-10 in HCV/HIV Co-infected Patients in China

OBJECTIVE To investigate expression and significance of CD25 and IP-10 in HCV mono-infection,HIV mono-infection and HCV/HIV co-infection.METHODS PBMC were isolated from patients with HCV infection(n=21),HIV infection(n=14),HCV/HIV co-infection(n=28) and normal control(n=30).The surface expression of CD25 on T lymphocytes was determined by flow cytometry.The serum IP-10 levels were measured by ELISA.RESULTS Increased levels of IP-10 were detected in HCV infection,HIV infection and HCV/HIV co-infection.A lower proportion of CD4+CD25+ T lymphocytes was found in HIV infection and HCV/HIV co-infection(P<0.01),while slight but not significant increased proportion of CD4+CD25+ T lymphocytes was detected in HCV infection.CONCLUSIONS Chemokine IP-10 and lymphocyte surface receptor CD25 are correlative with HCV and HIV infection closely.