| 贵州天麻种质资源的RAPD分析 |
| |
| 引用本文： | 邹佳宁,宋聚先,常楚瑞,王晓丽. 贵州天麻种质资源的RAPD分析[J]. 中药材, 2006, 29(9): 881-883 |
| |
| 作者姓名： | 邹佳宁 宋聚先 常楚瑞 王晓丽 |
| |
| 作者单位： | 贵阳医学院,贵州,贵阳,550004 |
| |
| 基金项目： | 科技部科研项目;贵州省科技攻关项目 |
| |
| 摘 要： | 对贵州省境内药用植物天麻的野生和栽培15个样品的基因组DNA遗传多态性的RAPD分析。从40个随机引物中筛选出12个有效引物,共检测到93个位点,其中66个显多态性,总的多态位点百分率(PPB)为70.97%。经NTSYSpc,ver.2.2软件进行UPGMA聚类分析,结果表明野生与栽培天麻之间的遗传变异较大,说明地理分布和生长环境的差异是导致天麻形成丰富的遗传多态性的一个重要的原因。
|
| 关 键 词： | 天麻 遗传多态性 |
| 文章编号： | 1001-4454(2006)09-0881-03 |
| 收稿时间： | 2006-01-16 |
| 修稿时间： | 2006-06-13 |
RAPD Analysis on the Germplasm Resources of Gastrodia elata in Guizhou |
| |
| ZOU Jia-ning,SONG Ju-xian,CHANG Chu-rui,WANG Xiao-li. RAPD Analysis on the Germplasm Resources of Gastrodia elata in Guizhou[J]. Journal of Chinese medicinal materials, 2006, 29(9): 881-883 |
| |
| Authors: | ZOU Jia-ning SONG Ju-xian CHANG Chu-rui WANG Xiao-li |
| |
| Affiliation: | Guiyang Medical Collge, Guiyang 550004, China |
| |
| Abstract: | Genetic polymorphisms of genomic DNA of 15 samples from wild and culturaled Gastrodia elata Blume in Guizhou were analyzed by RAPD method.12 effective primers are screened from 40 primers amplified a total number of 93 loci,among which 66 are polymorphic and the percentage of polymorphic loci(PPB) is 70.97%.UPGMA dendrogram analyzed by NTSYSpc,ver.2.2 shows that Gastrodia elata have apparent genetic variance.Geological distribution and growing environment were significant factors for the polymorphism. |
| |
| Keywords: | RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
