LHRH messenger RNA in neurons in the intact and castrate male rat forebrain,studied by in situ hybridization |
| |
| Authors: | J. M. Rothfeld J. F. Hejtmancik P. M. Conn D. W. Pfaff |
| |
| Affiliation: | (1) Departamento de Nutriçao, Universidade Federal de Pernambuco, Recife, PE, Brazil;(2) Departamento de Fisiologia, Universidade Federal de Pernambuco, Recife, PE, Brazil;(3) Departamento de Biofisica, Universidade Federal de Pernambuco, Recife, PE, Brazil;(4) Departamento de Neurobiologia, Universidade Federal Fluminense, Niterói, RJ, Brazil;(5) Department of Medical Physiology, The University of Calgary, 3330 Hospital Dr. N. W., T2N 4N1 Calgary, Alberta, Canada |
| |
| Abstract: | Summary The slow potential change (spc) accompanying spreading depression (SD) was studied in rats and in a seizure-sensitive strain of Mongolian gerbil under three different experimental paradigms, each involving the use of naloxone. Gerbils undergoing electroconvulsive shock treatment displayed SD during the post-ictal phase, which was blocked by the intraperitoneal (i.p.) administration of naloxone (20–50 mg kg-1). Topical application of naloxone to the exposed cortex of the anaesthetized gerbil and rat blocked the spc of SD evoked by KCl. Microiontophoretic ejection of naloxone during extracellular recordings reversed cell refractoriness following the spc, demonstrated by the observation of a maintained sensitivity to iontophoretic pulses of glutamate. The results suggest a possible involvement of naloxone-sensitive processes in the mechanism responsible for cortical SD. |
| |
| Keywords: | Slow potential change Spreading depression Cortex Naloxone Electroconvulsant shock Microiontophoresis |
| 本文献已被 SpringerLink 等数据库收录！ |
|
