抑制消减杂交技术克隆肺癌转移调控基因

目的 从一对高低转移表型有差异的人肺巨细胞癌细胞癌细胞株中分离并鉴定人类肿瘤转移抑制基因或具有转移抑制活性的核苷酸序列，为探讨肿瘤转移发生的分子生物学机制奠定基础。方法 使用抑制消减杂交技术，从一对同一亲本、转移表型不同的人肺巨细胞癌细胞株中分离转移抑制相关基因或核苷酸片段。结果 获得5个在低转移肺巨细胞中高表达的、均与已知的人类基因片段有很高同源性的核苷酸片段。结论 利用抑制消减杂交技术，从一对同源的高低转移表型差异的细胞株中获得了5条可能与肿瘤转移抑制相关的人cDNA序列，它们可能在维持肿瘤细胞自身稳定和防止肿瘤转移中起重要作用。

Suppression subtractive hybridization in the cloning of gene fragments in relation to lung cancer metastasis

Objective In order to provide important information about early diagnosis and treatment of tumor metastasis, a study on the biological regulation of metastasis at the molecular level was carried out by isolating and identifying human metastatic suppressor gene or related DNA sequences from a pair of cell clones with different metastatic phenotypes.Methods Suppression subtractive hybridization technique (SSH), gene cloning and analysis by bioinformatics were applied to this study. Two cell clones of pulmonary giant cell carcinoma originating from the same patient were used. The PLA 801C cell clone which shows lower metastatic potentiality was used as tester and the PLA 801D as a driver.Results Five cDNA clones so obtained were expressed on much higher levels in the poorly metastatic cancer cell clone than the highly metastatic one. Homology analysis of all five cDNA fragments by BLASTN through GenBank showed high homology with the known human genes.Conclusion Through using SSH, we have identified five cDNA fragments which are expressed at much higher levels in the poorly metastatic cancer cell clone than the highly metastatic variety. Therefore, it is supposed that they might play an important role in the suppression of metastatic tumor cells and may very well have potential application in the diagnosis and treatment of metastasis.