正交试验法优选黄芪中总黄酮提取工艺

目的：优选黄芪总黄酮的提取工艺条件。方法：采用正交试验法对黄芪总黄酮提取工艺条件进行优选，以总提物和总黄酮含量为指标，考察影响提取指标的4个因素，即溶媒用量（A），乙醇浓度（B），提取时间（C），提取次数（D）；每个因素取3个水平。结果：用乙醇提取黄芪总黄酮的最佳工艺为A2B2C1D2，即用10倍量70％乙醇回流提取2次，每次60min。结论：此法稳定可行，可为黄芪总黄酮提取工艺的确定提供依据。

Studing on Extraction Condition of Total Flavonoidsfrom Radix Astragali by Orthogonal Test Method

Objective： Select the best condition for extraction process of total flavonoids from Radix As- tragali. Method ： Regard the total extract yield and the yield of total flavonoids as indicators, total flavonoids extrac- tion conditions were optimized by using orthogonal test method. Four factors were examined by three levels： the a- mount of solvent （ A）, ethanol concentration （ B）, extraction time （ C）, extraction frequency （D）. Results ： The best extraction process of total flavonoids from Radix Astragali by ethanol is A2B2C1D2, that is, it was ex- tracted with 10 times the amount of 70% ethanol two times, 60min every time. Conclusion： The method can pro- vide a stable basis for extraction process of total flavonoids from Radix Astragali