麻黄汤及拆方对中性粒细胞趋化和释放白三烯的影响

目的:通过对麻黄汤(MHD)各拆方配伍组对中性粒细胞趋化和释放白三烯的抑制作用来探讨其配伍规律。方法:给大鼠腹腔注射糖原诱导中性粒细胞聚集,分离中性粒细胞,以花生四烯酸(arachidonic acid,AA),cal-ciumionophore(A₂₃₁₈₇)刺激离体中性粒细胞释放白三烯,再用HPLC法测定中性粒细胞中白三烯(LTB₄)的含量,并以fMLP为趋化因子,观察麻黄汤各拆方配伍组对脂多糖(LPS)刺激后的中性粒细胞趋化活性的影响。结果:麻黄汤及拆方对中性粒细胞趋化和释放白三烯的抑制作用不完全相同;麻黄汤及拆方也不同程度抑制中性粒细胞趋化和释放白三烯。结论:麻黄汤对中性粒细胞趋化和释放白三烯具有抑制作用,拆方分析显示麻黄汤全方效果最佳,并初步验证了麻黄汤组方的科学性和合理性。

Effects of compositions of Mahuang decoction on chemotaxis and leukotriene production from neutrophils in rats

Objective: To explore the regularity of recipe composition by observing inhibitory effects of disassembled compositions of Mahuang decoction (MHD) on chemotaxis and leukotriene production from neutrophils in rats. Method: Neutrophil aggregation was induced by in-traperitoneal injection of glycogen in rats. Intraperitoneal lavage fluid(PLF)was collected and neu-trophils were removed.Neutrophils were stimulated by calciumionophore A₂₃₁₈₇ in vitro to produce leukotriene B₄. The concentrations of leukotriene B₄ was measured by reversed-phase high performance liquid chromatography(HPLC) ,chemotatic chamber assay was used to investigate the regulative role of MHD on chenmotaxis of the neutrophils in response to LPS stimulation. Result: Disassembled compositions of MHD could inhibite chemotaxis and leukotriene production from neutrophils in rats. Inhibitory effects of MHD on mast cells were different. Conclusion: MHD has significantly inhibitory effects on chemotaxis and leukotriene production from neutrophils in rats. The original formula (MHD) works best. These results have confirmed the rationality and scientific level of MHD.