眼针对急性脑梗塞大鼠细胞凋亡及Bcl-2、Bax的影响

目的 从细胞凋亡及凋亡相关基因Bcl-2、Bax蛋白表达方面探讨眼针治疗急性脑缺血的作用机理。方法 用线栓法制成SD大鼠大脑中动脉阻塞再灌注模型，在阻塞2h，再灌注24h后，应用TUNEL法和免疫组织化学法分别对缺血对照组、缺血加眼针组、缺血加督脉电针组及假手术组大鼠脑组织凋亡细胞的分布及Bcl-2、Bax免疫反应阳性细胞进行观察。结果 眼针、督脉电针均可减少缺血所致DNA双链断裂(TUNEL阳性)，提高梗死周边区皮层Bcl-2／Bax的比值。结论 眼针、督脉电针对急性脑缺血的治疗作用可能通过抑制损伤而抑制细胞凋亡，从而保护脑神经细胞。

The Effect of Eye Acupuncture on Apoptosis,Bcl-2 and Bax in Acute Cerebral Infarction Rats

Objective To investigate the mechanism of eye-acupuncture treatment of acute cerebral ischemia from apoptosis and apoptosis-related gene Bcl-2 and Bax protein expressions. Methods SD rat's middle cerebral artery was occluded by thread insertion and reperfused 2 hr later. Twenty-four hours after the reperfusion, TUNEL method and immunohistochemical method were used to examine the distribution of apoptosis cells and Bcl-2 and Bax immunoreaction positive cells in the brain tissues in ischemia control, ischemia plus eye acupuncture, ischemia plus Du meridian electroacupuncture and sham operation groups of rats. Results Both eye acupuncture and Du meridian electroacupuncture could decrease the rupture of DNA double chains(TUNEL positive) due to ischemia and raise the ratio of Bcl-2 to Bax in the cortex in the periphery of the infarct.Conclusion Eye acupuncture and Du meridian electroacupuncture may produce a therapeutic effect on acute cerebral ischemia by reducing the injury, inhibiting apoptosis and protecting cerebral neurons.