慢性砷中毒对小鼠海马CA1区星形胶质细胞的影响

目的观察慢性砷中毒对成年小鼠海马CA1区星形胶质细胞的影响,探讨慢性砷中毒对成年小鼠脑部的神经毒性。方法选取健康成年昆明小鼠60只,雌雄各半,分为对照组、慢性砷中毒组,每组30只,分别以蒸馏水、1/10 LD50As2O3即As2O34.5 mg·kg^4.d^-1灌胃,连续3个月,根据其体重变化随时调整用药剂量。灌胃结束后,采用Y型电迷宫检测各组小鼠学习与记忆的功能,其后取小鼠海马组织,利用免疫组织化学技术检测砷中毒对小鼠海马CA1区胶质原纤维酸性蛋白（GFAP）表达的影响。结果与正常对照组比较,砷中毒组小鼠的学习、记忆能力明显低于对照组（P〈0.05）;免疫组化染色显示小鼠海马CA1区GFAP阳性细胞明显增多（P〈0.05）,阳性反应产物平均光密度增高（P〈0.05）。结论慢性砷中毒可引起小鼠海马CA1区星形胶质细胞反应性增生。

Effect of chronic arsenic poisoning on astrocyte in hippocampal CA1 area of mouse

Objective To observe the effect of chronic arsenic poisoning on astrocytes in the hippocampal CA1 area and to investigate the neurotoxicity of chronic arsenic poisoning in adult rats.Methods Totally 60 healthy adult Kunming mice were divided into chronic arsenic poisoning group and control group.1/10 LD50 As2O3 was given for 3 months by digestive tract（4.5 mg·kg^-4 ·d^-1） in chronic arsenic poisoning group while equal amont of distilled water was given in control group.The abilities of learning and memorizing were tested by Y-maze.The glial fibrillary acidic protein（GFAP）-positive cells in the hippocampal CA1 area were quantitatively analyzed with cell morphometric technique.Results Compared with control group,the changes in chronic arsenic poisoning group were： the ability of learning and memorizing were significantly lower than those in control group（P0.05）;the numbers and average optical density of GFAP-positive cells in the hippocampal CA1 area were increased than those of control group（P0.05）.Conclusion Chronic arsenic poisoning can increase the number of astrocyte in the hippocampal CA1 area.