老年骨关节炎患者关节软骨和滑膜中转化生长因子β及受体和金属蛋白酶组织抑制物的研究

目的 探讨骨关节炎（OA）患者关节软骨和滑膜中转化生长因子β（TGFβ1）、转化生长因子β受体（TGFβR）和金属蛋白酶组织抑制物－1（TIMP－1）变化及其与OA发病的关系。方法 采用免疫组化方法检测23例老年OA患者及3例外伤患者正常关节软骨和滑膜TGFβ1、转化生长因子βI类受体（TGFβRI）、转化生长因子βⅡ类受体（TGFβRⅡ）和TIMP－1的分布和阳性程度。结果 OA患者中14例关节软骨和16例滑膜TGFβ1染色呈阳性或弱阳性，14例关节软骨和15例滑膜TIMP－1染色呈弱阳性，全部OA患者关节软骨和滑膜TGFβRI和TGFβRⅡ染色呈强阳性。阳性细胞包括软骨细胞、滑膜衬里细胞、滑膜下层的血管内皮细胞和间质巨噬细胞等。结论 老年OA患者关节软骨和滑膜中TGFβ1、TGFβ及TIMP－1的变化可能与OA发病有一定的关系。

A study on transforming growth factor-beta, its receptor and tissue inhibitor of metalloproteinase-1 in the articular cartilage and the synovium of osteoarthritis in the elderly

Objective　To explore the changes of transforming growth factor-beta-1(TGFβ1), TGFβ receptor(TGFβR), and tissue inhibitor of metalloproteinase-1(TIMP-1), as well as their relations to the pathogenesis of elderly osteoarthritis. 　Methods　 The distribution and the levels of TGFβ1, TGFβ receptor Ⅰ(TGFβRⅠ) , TGFβ receptor Ⅱ(TGFβRⅡ) and TIMP-1 in the articular cartilage and the synovium of 23 aged patients with osteoarthritis and 3 patients with trauma(the joints were not injured) were examined using immunohistochemical methods. 　Results　 The immunohistochemical staining for TGFβ1 showed positive or weakly positive in the articular cartilage and the synovium in two thirds of the patients with osteoarthritis. The strongly positive staining for TGFβRⅠ and TGFβRⅡ was found in the articular cartilage and the synovium in all of the osteoarthritis patients. The immunohistochemical staining of TIMP-1 showed weakly positive in the articular cartilage and the synovium of two thirds of patients with osteoarthritis. The positively stained cells containing TGFβ1, TGFβRⅠ, TGFβRⅡ and TIMP-1 included chondrocytes, synovial lining cells, endothelial cells and the macrophages in subsynovial layer with osteoarthritis. 　Conclusions　 The distribution and the content of TGFβ1, TGFβR and TIMP-1 in the articular cartilage and the synovium of osteoarthritis in the elderly are considered to correlate with the pathogenesis of osteoarthritis.