康莱特注射液诱导人肝癌细胞凋亡的研究

目的：探讨康莱特注射液诱导人肝癌细胞凋亡的作用。为康莱特注射液治疗肝癌提供理论依据。方法：采用免疫组织化学及透射电镜技术观察康莱特注射液对肝癌细胞凋亡的影响。结果：肝癌细胞经康莱特注射液培养24h后，其凋亡的百分率明显高于对照组和空白组，有显著性差异（P＜0．05），且组织细胞凋亡在50％以上者占44．12％（15／34），而对照组及空白组无1例凋亡超过50％；电镜相可见较为典型的细胞凋亡的形态学变化，细胞核固缩，染色质凝集，呈新月型紧贴于核膜周边，核碎裂，凋亡小体形成等。结论：康莱特注射液能诱导肝癌细胞发生凋亡；诱导细胞凋亡可是康莱特注射液治疗作用的重要机制。

Apoptosis of Human Hepatocellular Carcinoma Cells Induced by Kanglaite

Objective To investigate the effect of Kanglaite injection in inducing apoptosis of human hepatocellular carcinoma cells.Methods The hepatocellular carcinoma cells,isolated from 34 surgical specimens of hepatocellular carcinoma,were cultured with kanglaite for 24 hours in vitro.The apoptosis of the cells was observed by immunohistochemical method and electromicroscope.Results The apoptosis rate in kanglaite group was significantly higher compared with control(P<0.05).The characteristics of apoptosis such as the formation of cell-membrane blebs and apoptotic bodies,were observed morphologically by electromicroscope.Conclusion Kanglaite injection could induce apoptosis of hepatocellular carcinoma.The induction apoptosis may be the major mechanism in therapy of hepatocellular carcinoma with Kanglaite injection.