尿毒症腹膜透析模型中淋巴孔超微结构的观察

目的:观察在尿毒症腹膜透析条件下淋巴孔超微结构的改变.方法:采用SD雄性大鼠制作尿毒症腹膜透析模型,试验分为4组,正常组、尿毒症组、1.5%尿毒症腹膜透析组、4.25%尿毒症腹膜透析组,在实验的第1周及第4周检测肾功能及血浆白蛋白,并且应用扫描电镜观察不同分组的膈肌组织中淋巴孔超微结构的改变.结果:在两组尿毒症腹膜透析组及尿毒症组中,其淋巴孔直径明显增大,与正常组相比较,有统计学差异.结论:尿毒症腹膜透析模型中淋巴孔出现明显改变,可能与腹膜超滤受损有关.

Ultrastructural Changes of Stomata in the Uremic Rats Undergoing Perioneai Dialysis

Objective:To investigate the ultrastructural changes of stomata in the uremic rats undergoing peritoneal dialysis. Methods:The study was done in male SD rats, Animals were divided into four groups: normal rats (group 1) and 5/6 nephroectomy uremic rats ( group 2) and dialysized uremic rats exposed to PD solution 4.25%( group 3) and 1.5%( group 4)respectively, serum creatinine and blood urea nitrogen and plasma albumin were tested in the first week and the fourth week. In the end of the study, all rats were sacrificed and the diaphragms were taken to investigate the ultrastructural changes of stomata by electronic scan microscope, and the diameters of the stomata were analyzed by software ImageTool. Results:The diameters of stomata in uremic rats and uremic rats undergoing peritoneal dialysis were remarkablely dilated to compare to that of normal rats,(P<0.05 ). Conclusion:Abnormal changes of the diameters of the stomata in uremic rats undergoing peritoneal dialysis were associated to the ultrafiltration disfunction in peritoneal dialysis.