Chemical composition and protective effect of Juniperus sabina L. essential oil against CCl4 induced hepatotoxicity |
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| Authors: | Maged S. Abdel-Kader Gamal A. Soliman Mohammed H. Alqarni Abubaker M. Hamad Ahmed I. Foudah Saleh I. Alqasoumi |
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| Affiliation: | 1. Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia;2. Department of Pharmacognosy, College of Pharmacy, Alexandria University, Alexandria 21215, Egypt;3. Department of Pharmacology, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia;4. Department of Pharmacology, College of Veterinary Medicine, Cairo University, Giza, Egypt;5. Basic Sciences Department, Preparatory Year Deanship, Prince Sattam Bin Abdulaziz University, PO Box 20337, Alkharj 11942, Saudi Arabia;6. Department of Histopathology and Cytopathology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Madani, Sudan;7. Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia |
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| Abstract: | The hepatoprotective activity of the total extract of Juniperus sabina L. against CCl4 induced toxicity in experimental animals was previously reported and indicated promising results. Essential oil of J. Sabina was prepared by hydrodistillation method. Components of the oil were identified by comparison of GC-MS and retention indexes with reported data. The hepatoprotective effect of the essential oil against CCl4 induced toxicity was studied using male Wistar rats and silymarin at 10 mg/kg p.o as standard drug. The protective effect was evaluated via serum biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyltranspeptidase (GGT), and total bilirubin as well as tissue parameters including non-protein sulfhydryl groups (NP-SH), malonaldehyde (MDA) and total protein (TP). Histopathological study was applied on the liver tissues using Mayer’s hematoxylin stain, Periodic Acid Schiff – Hematoxylin (PAS-H) and Masson trichrome technique on light microscope. Electron microscope images were also obtained for more detailed study. |
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| Keywords: | Corresponding author at: Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, 11942 Alkharj, Saudi Arabia. Hepatoprotective Essential oil Biochemical parameters Electron microscope |
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