RP-HPLC法测定人血清中阿莫西林浓度及应用

目的：建立测定人血清中阿莫西林浓度的RP—HPLC法。方法：采用甲醇沉淀蛋白，高效液相色谱检测法，以别嘌醇为内标，色谱柱为Phenomenex Synergi4μPOLAR-80A，250mm×4．6mm，流动相为甲醇-0．2％甲酸水溶液（含10mmol·L^-1乙酸铵）／4：96，流速1．2mL·min^-1，检测波长：236nm。结果：血清中内源性物质不干扰阿莫西林的检测，阿莫西林的线性范围为0．327～20．95／2g·mL^-1，批内、批间精密度均小于15％，低、中、高浓度的（0．655、2．619和10．475μg·mL^-1）平均提取回收率分别为102．9％、99．8％、97．6％。结论：该法操作快速、简单、准确，符合生物样品检测要求，可用于临床药代动力学研究中大批量血样的处理。

Determination of Serum Concentration of Amoxicillin by RP-HPLC

OBJECTIVE： To develop a RP- HPLC method for determination of amoxicillin in human serum. METHODS： Serum protein was precipitated with methanol. With antigut used as internal standard, the analysis was conducted on Phenomenex Synergi 4 μ POLAR - 80A （250 mm×4.6 mm） column. The mobile phase consisted of Methanol - 0.2 % formic acid （containing 10 mmol·L^-1 ammonium acetate） at a flow rate of 1.2 mL·min^-1 and a detection wavelength of 236 nm. RESULTS： The detection was free of interference from serum endogenous substance. The linear concentration range of Amoxicillin was 0. 327 - 20.95 μg·mL^-1. The intra - and inter- day precision over the entire concentration range were less than 15%. The mean extraction recoveries of amoxicillin at concentrations of 0.655, 2.619 and 10.475μg·mL^-1 were 102.9%, 99.8% and 97.6%, respectively. CONCLUSION： The method is rapid, simple and accurate, meeting the standard for the detection of biological specimen, and it is applicable for the treatment of a large number of blood samples in the research of clinical pharmacokinetics.