丹参多酚酸盐对2型糖尿病大鼠心脏保护作用

目的探讨丹参多酚酸盐对2型糖尿病大鼠心脏保护作用。方法选择30只健康雄性Wistar大鼠中的20只，以高糖高脂饲料喂养6周后腹腔注射链脲佐菌素30mg／kg，3d后测血糖≥17．8mmol／L（n=17）入选糖尿病大鼠模型，并随机分为糖尿病组（n=9）、丹参多酚酸盐（15mg／kg）治疗组（n=8），另外10只大鼠为对照组（n=10）。治疗组给予丹参多酚酸盐治疗8周，彩色多普勒超声检测大鼠心功能，光镜及透射电镜观察心肌病理学改变，转移酶介导的缺口末端标记法检测心肌细胞凋亡，荧光定量聚合酶链反应检测心肌细胞Bcl-2及半胱氨酸天冬酶3（Caspase-3）基因的表达情况。结果与对照组相比．糖尿病组凋亡心肌细胞明显增多（14．57±0．56）比（0．87±0．13）]（P〈0．05）；心肌中Bcl-2基因表达减弱（0．57±0．05）比（1．06±0．09）]（P〈0．05）；Caspase-3基因表达增强（1．94±0．15）比（1．02±0．08）]（P〈0．05）；与糖尿病组相比，丹参多酚酸治疗组凋亡心肌细胞减少（6．16±0．64）]（P〈0．05）；心肌Bcl-2基因表达增多（0．84±0．09）]（P〈0．05）；Caspase-3基因表达减少（1．37±0．13）]（P〈0．05）。结论应用丹参多酚酸盐治疗后，可延缓2型糖尿病心肌病变发展，保护心脏。

Protection effect of salvianolate in type 2 diabetes rats

Objective To explore the protection effect of salvianolate in type 2 diabetes rats. Methods Of 30 healthy male Wistar rats, 20 were selected and fed high-fat and high-sucrose diet for 6 weeks. They were injected intraperitoneally streptozotocin（ STZ, 30 mg/kg）. After 3 days, the rats with plasma glucose ≥17.8 mmol/L were as the rat model of diabetes （n = 17） and randomly divided into the diabetes group（ n = 9） and salvianolate （15 mg/kg） treatment group （n =8）. Other 10 heathy male wistar rats were included into the control group（group N,n = 10）. S group were treated with salvianolate by intraperitoneal injection for 8weeks. N and D groups rats were given saline. Eight weeks later, heart function was detected by Doppler eehocardiography; the pathological changes were observed under transmission electron microscope and light microscopy; the levels of Bcl-2 and Caspase-3 gene expressions were examined by fluorescence quantitative polymerase chain reaction. Results Compared with control group, car- diac myoeyte apoptosis was significantly increased （ 14.57 ± 0.56 ） vs （ 0.87 ± 0.13 ） （ P 〈 0.05 ） ; Bcl-2 expression was decreased（0.57 ± 0.05） vs （ 1.06 ± 0.09） （P 〈 0.05） ; Caspase-3 expression was significantly increased （ 1.94 ± 0.15 ） vs （ 1.02 ± 0.08 ） （ P 〈 0.05 ）. Compared with diabetes group, after given salvianolate treatment, apoptotic myocardial cells decreased （6.16 ±0.64） vs （14.57±0.56） ] （P 〈0.05） ;the level of myocardial Bel-2 gene expression increased （0.84 ±0.09） vs （0.57 ±0.05） ] （P 〈0.05） ;while Caspase-3 decreased （ 1.37 ± 0. 13 ） vs （ 1.94 ± 0.15 ） ] （ P 〈 0.05 ）. Conclusion Salvianolate treatment can delay the development of type 2 di- abetic eardiomvoDathv and protect the heart.