重度妊娠高血压综合征患者胎盘组织细胞凋亡及其调控基因 …

目的　研究细胞凋亡及其调控基因在重度妊娠高血压综合征 (妊高征 )发生、发展中的作用。方法　对 40例正常晚孕妇女 (对照组 )和 40例重度妊高征患者 (观察组 )的胎盘组织进行分析。用DNA缺口原位末端标记 (TUNEL)技术检测细胞凋亡 ;免疫组织化学方法检测促进和抑制凋亡基因 (bax/bcl 2 )的表达。结果　对照组胎盘细胞滋养细胞、合体滋养细胞中凋亡指数分别为 ( 1.1±0 .9) %、( 41.8± 1.5 ) % ;bax阳性率分别为 ( 1.0± 0 .9) %、( 2 8.9± 9.7) % ;bcl 2阳性率分别为 ( 2 .2±0 .8) %、( 2 2 .9± 0 .7) % ,总bax/bcl 2为 0 .7～ 1.7。观察组胎盘细胞滋养细胞、合体滋养细胞的凋亡指数分别为 ( 4.3± 1.2 ) %、( 45 .3± 1.4) % ;bax阳性率分别是 ( 2 .2± 0 .8) %、( 42 .5± 11.7) % ;bcl 2阳性率分别是 ( 3 .2± 0 .8) %、( 2 3 .3± 7.8) % ;总bax/bcl 2为 1.0～ 3.2。即 :对照组胎盘中有一定量的细胞凋亡、bax、bcl 2表达 ,bax/bcl 2表达间呈平衡趋势 ;观察组中细胞凋亡明显增高 (P <0 .0 1) ,bax表达也明显增强 (P <0 .0 1) ,bcl 2表达仅呈增高趋势 ,但差异无显著性 (P >0 .0 5 ) ,bax/bcl 2比值明显增高(P <0 .0 1)。结论　细胞凋亡及其调节基因的表达间具有一致性 ;细胞凋亡、bax/bcl 2表达

Study on Apoptosis and its Related Genes in Pregnancy Induced Hypertension

OBJECTIVE: To investigate apoptosis and its related genes expression in serious pregnancy induced hypertension (SPIH). METHODS: 40 cases of normal term pregnancy(NTP) and 40 cases of SPIH were enrolled in this study. Apoptosis was detected using DNA in situ terminal deoxynucleotidyl transferas (TDT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick ending labeling (TUNEL); bax and bcl-2 were detected by immunohistochemical staining (SP). RESULTS: In NTP group apoptosis index(AI) of cytotrophoblasts(CT) and syncytiophoblasts(ST) in placenta was (1.1 +/- 0.9)% and (41.8 +/- 1.5)%; bax positive rate was (1.0 +/- 0.9)% and (28.9 +/- 9.7)%; bcl-2 was (2.2 +/- 0.8)% and (22.9 +/- 0.7)% respectively; bax/bcl-2 was 0.7-1.7. In SPIH group, AI was (4.3 +/- 1.2)% and (45.3% +/- 1.4)%; bax was (2.2 +/- 0.8)% and (42.5 +/- 11.7)%; bcl-2 was (3.2 +/- 0.8)% and (23.3 +/- 7.8)%; bax/bcl-2 was 1.0-3.2. The difference between SPIH and NTP was significant respectively (P < 0.01); bax/bcl-2 was balance in NTP, while the balance was broken in SPIH. CONCLUSIONS: Apoptosis and its related genes expression are simultaneous. The balance and imbalance of apoptosis and bax/bcl-2 affect the structure and the function of placenta, and then may further affect the outcome of pregnancy.