急性白血病患者VEGF水平测定及意义探讨

目的探讨VEGF水平和白血病类型、病情的关系,为白血病的治疗和预后的判断提供依据。方法测定不同病程时期急性淋巴细胞白血病(ALL)和急性非淋巴细胞白血病(ANLL)外周血、骨髓和骨髓有核细胞培养上清液中VEGF水平,同时检测骨髓幼稚细胞水平,分析VEGF与白血病类型、病情及预后的关系。结果ALL组和ANLL组的不同病程时期,外周血、骨髓血及骨髓细胞培养上清液VEGF水平均明显高于对照组,ALL和ANLL组间差别无显著性意义。同一组内,骨髓血VEGF水平高于外周血,外周血高于骨髓细胞培养上清液。急性白血病初诊、缓解、难治/复发各组外周血VEGF水平均高于对照组(P值均<0.01)。其中以例难治/复发者最高。化疗缓解后VEGF水平下降,但仍高于对照。白血病患者外周血VEGF水平与骨髓原幼细胞比例变化一致。结论外周血、骨髓VEGF水平与白血病病情正相关,对白血病的诊断、治疗和预后的判断有一定意义。

Significance of Vascular Endothelial Growth Factors in Patients With Acute Leukemia

Objective To investigate the correlation between the level of vascular endothelial growth factors(VEGF), type and disease staging in patients with acute leukemia. Methods To detect VEGF in peripheral blood, bone marrow blood and cultured bone marrow nucleared cells supernatant from different stage of acute lymphocyte leukemia(ALL) and acute nonlymphocyte leukemia(ANLL) patients. The rate of blast cells and juvenile cells in patients'marrow were assessed simultaneously and analyzed statistically. Results VEGF in peripheral blood, bone marrow blood and cultured bone marrow nucleared cells supernatant from different stage of ALL and ANLLL patient groups were significantly higher than that from the control group, but not between ALL group and ANLL group. The VEGF decreased after remission, but it was still higher than that from the control group. The recidivist group processed a highest VEGF level in all groups. The VEGF level and rate of blast cells and juvenile cells in patient' bone marrow cells showed positive correlation. Conclusion Assessment of VEGF for diagnosis and estimation of prognosis in patients with acute leukemia is of important clinical significance.