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| 乙型肝炎病毒表面抗原118位氨基酸变异研究 | |
| 引用本文： | 杨永林,孟钵,马贵明,赵静,黄祖瑚. 乙型肝炎病毒表面抗原118位氨基酸变异研究[J]. 中国输血杂志, 2009, 22(10) |
| 作者姓名： | 杨永林 孟钵 马贵明 赵静 黄祖瑚 |
| 作者单位： | 1. 南京红十字血液中心,江苏,南京,210003;南京医科大学第一附属医院 2. 南京红十字血液中心,江苏,南京,210003 3. 南京医科大学第一附属医院 |
| 摘 要： | 目的研究献血者乙型肝炎病毒表面抗原118位氮基酸变异对ELISA检测的影响。方法PCR扩增献血者表面抗原基因,恢复变异点氨基酸为野生型,构建不同的表达载体,在293T细胞株中表达,检测各自在不同试剂中的反应格局。结果117位点对检测反应格局不产生明显影响,118位点的变异明显影响检测格局,恢复变异点氨基酸明显提高了检出率和灵敏度。结论118位氮基酸变异影响了乙肝表面抗原的筛查,改变了其的免疫反应原性。 |
| 关 键 词： | 乙型肝炎病毒表面抗原 主要亲水区 酶联免疫吸附试验 献血者 |
Effect of HBsAg mutation at aa118 position on the HBsAg blood screening test |
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| YANG Yonglin,,MENG Bo,MA Guiming,et al.. Effect of HBsAg mutation at aa118 position on the HBsAg blood screening test[J]. Chinese Journal of Blood Transfusion, 2009, 22(10) | |
| Authors: | YANG Yonglin MENG Bo MA Guiming et al. |
| Affiliation: | YANG Yonglin1,2,MENG Bo1,MA Guiming1,et al. 1 Nanjing Blood Center,Nanjing 210003,China,2 The First Affiliated Hospital with Nanjing Medical University |
| Abstract: | Objective To study the effect of HBsAg polymorphism at aa118 position on the blood HBsAg screening test.Methods HBsAg gene,amplified by PCR,was cloned and sequenced,then mutated amino acids were recovered into the wild type at aa117 or/and aa118 position. By the insertion of HBsAg genes,expression vectors were constructed and transient expressions were committed in 293T cells. Suspensions of cell culture were collected and tested by the panel,which contained 10 kinds of HBsAg commercial testing kits.Result ... |
| Keywords: | Hepatitis B Surface Antigen Major Hydrophilic Region Mutation Enzyme linked immunosorbent assay Blood donation |
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