| 人精子质膜孕激素受体研究 |
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| 引用本文： | 霍涌玮,邱曙东,许拥军,成少利,武捷,王丽蓉,葛玲. 人精子质膜孕激素受体研究[J]. 中华男科学杂志, 2002, 8(4): 277-280 |
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| 作者姓名： | 霍涌玮 邱曙东 许拥军 成少利 武捷 王丽蓉 葛玲 |
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| 作者单位： | 1. 西安交通大学医学院组织学和胚胎学教研室,陕西,西安,710061
2. 陕西省新安中心医院,陕西,西安,710048 |
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| 摘 要： | 目的 :观察人精子表面孕激素受体 (PR)的定位及阳性表达率。 方法 :精子体外获能后 ,应用异硫氰酸荧光素标记的牛血清白蛋白 孕酮复合物 (P BSA FITC)染色 ,荧光显微镜观察及流式细胞术 (FCM )定量分析法 ,分别观察与孕酮 (P)结合精子形态及标记精子所占比例。 结果 :P BSA FITC染色精子的形态主要表现为 2种类型 :整个顶体区域或仅赤道区呈绿色荧光 ,顶体后区及尾部不着色。与P结合精子的百分率为 (30 2± 2 4 ) %。 结论 :人精子顶体表面有PR表达 ,且这种表达呈选择性
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| 关 键 词： | 精子 孕激素受体 人 |
| 文章编号： | 1009-3591(2002)04-0277-04 |
| 修稿时间： | 2002-06-08 |
Investigation of Progesterone Receptor on Human Sperm Plasma Membrane |
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| Yong Wei HUO ,Shu Dong QIU ,Yong Jun XU ,Shao Li CHENG ,Jie WU ,Li Rong WANG ,Ling GE. Investigation of Progesterone Receptor on Human Sperm Plasma Membrane[J]. National journal of andrology, 2002, 8(4): 277-280 |
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| Authors: | Yong Wei HUO Shu Dong QIU Yong Jun XU Shao Li CHENG Jie WU Li Rong WANG Ling GE |
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| Affiliation: | Department of Histology and Embryology, Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China. |
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| Abstract: | OBJECTIVES: To investigate the localization and positive percentage of progesterone receptor (PR) on the human sperm surface. METHODS: After in vitro capacitation, progesterone binding sites on the sperm were quantitatively analyzed by fluorescence microscopy and flow cytometry using fluorescein isothiocyanate-labeled bovine serum albumin-progesterone complex (P-BSA-FITC). RESULTS: The spermatozoa stained by P-BSA-FITC mainly showed two labeling patterns, with the green fluorescence on the whole acrosomal region or the equatorial acrosomal region only and the stainless postacrosomal and tail regions. The percentage of progesterone-binding sperm was (30.2 +/- 2.4)%. CONCLUSIONS: There is selective expression of PR on the human sperm acrosome surface. |
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| Keywords: | Sperm Progesterone receptor Human |
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