黄芩苷对大鼠脑缺血再灌注损伤的保护作用

目的探讨黄芩苷对局灶性脑缺血再灌注损伤的保护作用及其机制。方法线栓法制备大鼠大脑中动脉局灶性脑栓塞模型,缺血2h,再灌注24h。评价神经功能状态,测定脑梗塞体积和丙二醛(MDA)和一氧化氮(NO)含量、超氧化物歧化酶(SOD)和髓过氧化物酶(MPO)活性以及血脑屏障通透性。称重法测定组织脑水肿含量。结果黄芩苷能显著降低缺血再灌注后脑梗塞体积,改善神经功能状态,降低脑组织MDA、NO含量、血脑屏障通透性和脑水肿程度,升高SOD活性并降低MPO活性,与模型组比较,具有显著性差异(P<0.05或P<0.01)。结论黄芩苷对大鼠脑缺血再灌注损伤具有明显的保护作用,其机理与其清除氧自由基、抗炎和减轻脑水肿有关。

Protective Effect of Baicalin on Cerebral Ischemia Reperfusion Injury in Rats

Objective To investigate the protective effect of baicalin on cerebral injury induced by transient focal ischemia and its mechanisms.Methods Transient focal cerebral isehemia injury model in rats was induced by occlusion of the right middle cere- bral artery for 2 h,followed by 24 h reperfusion.The infarction volume and neurological deficit determined by the methods of TTC staining and the Longa's score were used to evaluate the effect of baiealin on cerebral injury.The levels of mallondialdehyde (MDA),nitric oxide(NO),activities of superoxide dismutase(SOD)and myeloperoxidase(MPO)and blood-brain barrier (BBB)permeability in brain were measured by spectrophotometer.The content of brain water was assayed by weighing method. Results Pretreatment with baicalin markedly reduced brain infarction volume and neurological deficit induced by transient ische- mic insult,inhibited MPO activity,decreased NO,MDA and brain water content,and BBB permeability,increased SOD activi- ty.Conclusion Baicalin might play a protective effect on cerebral ischemia reperfusion injury through inhibiting inflammatory process,peroxidation and decreasing brain water contents.