自体细胞因子诱导的杀伤细胞联合常规化疗治疗中晚期宫颈癌的临床研究

目的 探讨回输自体细胞因子诱导的杀伤细胞(CIK)联合顺铂治疗中晚期宫颈癌的临床疗效,并观察此治疗对患者外周血中Th1/Th2细胞因子的漂移的影响.方法 将我院在2013年2月至2014年2月接收的宫颈癌患者44例,随机分为单纯化疗组(n=22)和联合治疗组(n=22),并选取健康的女性个体20例作为对照组,采用酶联免疫吸附(ELISA)法检测检测所有样本外周血中Th1型细胞因子白细胞介素(IL)-2、干扰素-γ(IFN-γ)的表达水平,Th2型细胞因子IL-4、IL-6、IL-10的表达水平,流式细胞仪检测Th1/Th2细胞的比率变化.同时评价两组患者疗效及生活质量.结果 与健康对照组比较,治疗后两组患者血清中Th1型细胞因子IL-2、IFN-γ的浓度显著升高,且联合治疗组升高程度高于单纯化疗组,而Th2型细胞因子IL-4、IL-6、IL-10的浓度明显降低,且联合治疗组降低程度高于单纯化疗组(P＜0.05).治疗后单纯化疗组和联合治疗组Th1/Th2比率小于对照组,但联合治疗组高于单纯化疗组(P＜0.05).联合治疗组完全缓解率高于单纯化疗组(36.4％比22.7％,P＜0.05).联合治疗组有效率高于单纯治疗组(90.9％比72.7％,P＜0.05).联合治疗组的生活质量总提高率高于单纯化疗组(86.4％比72.7％,P＜0.05).结论 自体CIK细胞联合常规化疗能够改善中晚期宫颈癌的Th1向Th2漂移,是安全并且有效的.

Clinical study of autologous cytokine induced killer cells combined with chemotherapy for patients with advanced cervical cancer

Objective To explore the clinical effectiveness of autologous cytokine induced killer cells (CIK) combined with chemotherapy in treatment of patients with advanced cervical cancer and observe the influence on the switching of Th1/Th2 cytokines.Methods 44 cervical carcinomapatientsl from January 2013 to January 2014 were randomly divided into chemotherapy group(n =22)and combined group(n =22),20 healthy controls were recruited at the same time.Th1 cytokines [interleukin (IL)-2,interferon (IFN)-γ] and Th2 cytokines(IL-4、IL-6 、IL-10)in peripheral blood were detected by ELISA kit,and the Th1/Th2 subpopulations changes was detected by flow cytometry,the quality of life and effectiveness were compared too.Results Compared with control group,the levels of Th1 cytokines (IL-2,IFN-γ) decreased,while the levels of Th1 cytokines (IL-4,IL-6,IL-10)significantly increased,and the changes of the combined treatment group were significantly higher than those of chemotherapy group(P ＜ 0.05).Both the ratio of Th1/Th2 cells in therapy groups were significantly less than the control group,but the combined treatment group was significantly higher than that of chemotherapy group (P ＜ 0.05).The complete remission rate (36.4％ vs 22.7 ％),effectiveness (90.9％ vs 72.7％),and life quality(86.4％ vs 72.7％)of combination treatment group were significantly higher than those of chemotherapy group(P ＜ 0.05).Conclusion The switching of Th1/Th2 cytokines could be improved by CIK cells in combination with standard chemotherapy for patients with advanced cervical cancer.It is concluded that autologous CIK cells combined with chemotherapy is safe and efficacious.