Immunochemical Characterization of a Monoclonal Anti-Leb Blood Grouping Reagent |
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| Authors: | L. Messeter T. Brodin M.A. Chester K.-A. Karlsson D. Zopf and A. Lundblad |
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| Affiliation: | Department of Clinical Chemistry, Lund, Sweden;Blood Bank, University Hospital, Lund, Sweden;Department of Tumor Immunology, Wallenberg Laboratory, Lund, Sweden;Department of Medical Biochemistry, University of Gothenburg, Sweden;National Cancer Institute, The National Institutes of Health, Bethesda, Md., USA |
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| Abstract: | ![]()
A haemagglutinating monoclonal anti-LebL antibody has been obtained from a mouse-mouse hybridoma after immunization with the Leb-active oligosaccharide lacto-N-difucohexaose I, coupled to edestin. Of 13 other antibodies obtained, one was able to agglutinate enzyme-treated erythrocytes of all ABO groups and three more were considered to be anti-LebH haemagglutinins. The remaining nine antibodies did not agglutinate red cells. The specificity of the stronger of the anti-LebL haemagglutinins was examined by studying its binding to a number of glycoconjugates and oligosaccharides. |
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