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Imaging Trypanosoma cruzi within tissues from chagasic patients using confocal microscopy with monoclonal antibodies |
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| Authors: | Renato Arruda Mortara Solange da Silva Francy R. Patrício Maria de Lourdes Higuchi Edison Reis Lopes Alberto Alain Gabbai Paola Carnevale Ademir Rocha Marcelo S. Ferreira Márcia M. Souza Marcelo F. de Franco Gilberto Turcato Jr. Ben-Hur Ferraz Neto |
| Affiliation: | Disciplina de Parasitologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de S?o Paulo, Escola Paulista de Medicina, R. Botucatu, 862, 6° andar, 04023-062, S?o Paulo, SP, Brazil e-mail: renato.dmip@epm.br, Tel.: +55-11-5708306, Fax: +55-11-5711095, BR Departamento de Anatomia Patológica, Universidade Federal de S?o Paulo, Escola Paulista de Medicina, Instituto do Cora??o, Faculdade de Medicina USP, S?o Paulo, SP, Brazil, BR Faculdade de Medicina do Triangulo Mineiro, Uberaba, MG, Brazil, BR Disciplina de Neurologia, Universidade Federal de S?o Paulo, Escola Paulista de Medicina, Disciplina de Cardiologia, Universidade Federal de S?o Paulo, Escola Paulista de Medicina, Centro de Ciências Biomédicas, Universidade Federal de Uberlandia, Uberlandia, MG, Brazil, BR Departamento de Moléstias Infecciosas e Parasitárias, Universidade Federal de S?o Paulo, Escola Paulista de Medicina, Unifígado, Hospital Alem?o Oswaldo Cruz, S?o Paulo, SP, Brazil, BR |
| Abstract: | Confocal fluorescence microscopy combined with differential interference contrast imaging of tissues from chagasic patients enabled the unequivocal identification of the parasite Trypanosoma cruzi. Using different monoclonal antibodies that indicate the parasite form and replication stage in conjunction with DNA labelling, specimens derived from distinct clinical forms of the disease were examined. Intracellular amastigote forms of the parasite were clearly detected in heart, brain, skin, lung, and kidney. Dividing amastigotes as well as trypomastigote forms were recognized in samples obtained from patients undergoing either acute-phase or some form of reactivation caused by immunosuppression. Received: 22 December 1998 / Accepted: 11 March 1999 |
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