| 阿霉素肾病大鼠nephrin及podocalyxin蛋白表达及泼尼松干预作用的探讨 |
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| 引用本文： | 胡小云,张碧丽.阿霉素肾病大鼠nephrin及podocalyxin蛋白表达及泼尼松干预作用的探讨[J].山东医药,2011,51(40):57-60,F0003. |
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| 作者姓名： | 胡小云 张碧丽 |
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| 作者单位： | 1. 天津医科大学研究生院,天津,300070
2. 天津市儿童医院 |
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| 基金项目： | 天津市卫生局科技基金项目(03KY32) |
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| 摘 要： | 目的 探讨阿霉素肾病（ADN）大鼠肾组织nephrin及podocalyxin蛋白表达及泼尼松的干预作用。方法 24只雄性Wistar大鼠随机分成ADN组、泼尼松组、对照组，于0、4、8周测定大鼠24h尿蛋白（24hUP）、血清胆固醇（Cho）及白蛋白（Alb）。实验8周取肾组织观察病理形态改变，计算肾小球细胞外基质面积与肾小球面积之比（ECM/GA），评估肾脏病理积分。用免疫组织化学法观察肾小球nephrin、podocalyxin表达。结果 实验4周时，ADN组较之对照组24hUP、Cho升高（P〈0.05），Alb下降（P〈0.01）。实验8周时，泼尼松组较ADN组24hUP、Cho下降，ECM/GA及肾脏病理积分降低，nephrin、podoralyxin表达增多，Alb升高（P〈0.01）。ADN组nephrin、podlocalyxin表达与24hUP、Cho、ECM/GA及肾小球病理积分均呈负相关，与Alb呈正相关；nephrin与podocalyxin呈正相关。结论 nephrin、podocalyxin与蛋白尿的发生及发展密切相关。泼尼松能减轻ADN大鼠的蛋白尿及肾脏病理损害，其机制可能通过上调nephrin、podoralyxin表达来发挥肾保护作用。
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| 关 键 词： | 泼尼松 阿霉素肾病 nephrin podocalyxin |
Expression of nephrin and podocalyxin in rats with adriamycin-induced nephrosis and intervention effect of prednisone |
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| HU Xiao-yun,ZHANG Bi-li.Expression of nephrin and podocalyxin in rats with adriamycin-induced nephrosis and intervention effect of prednisone[J].Shandong Medical Journal,2011,51(40):57-60,F0003. |
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| Authors: | HU Xiao-yun ZHANG Bi-li |
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| Institution: | HU Xiao-yun1,ZHANG Bi-li(1 Graduate School,Tianjin Medical University,Tianjin 300070,P.R.China) |
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| Abstract: | Objective To investigate the expression of nephrin and podocalyxin in rats with adriamycin-induced nephrosis(ADN) and the intervention effect of prednisone.Methods Twenty-four male Wistar rats were randomly divided into three groups,which were ADN group,prednisone group,control group.Serum cholesterol,albumin(Alb),and the 24-hour urine protein(24hUP) were measured serially in the 0,4th,and 8th week.Rats were sacrificed to collect kidney tissue for pathological morphology observation in the end of study(the ... |
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| Keywords: | prednisone adriamycin-induced nephrosis nephrin podocalyxin |
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