CD81和趋化因子CC受体5与丙型肝炎合并人类免疫缺陷病毒感染的相关性研究

目的 检测外周血CD4＋及CD8＋T淋巴细胞表面CD81、CCR5的表达,探讨与丙型肝炎病毒（HCV）单纯感染、人类免疫缺陷病毒（HIV）单纯感染和HCV/HIV合并感染的关系.方法 采用流式细胞术,检测HCV感染组（n=21）、HIV感染组（n=14）、HCV/HIV感染组（n=28）及正常对照组（n=30）人外周血CD4＋及CD8＋T淋巴细胞表面CD81和CCR5的表达.结果 与正常对照组相比,外周血CD4＋T淋巴细胞表面CD81,在HCV感染组表达变化不明显,而在HIV感染组和HCV/HIV合并感染组中低表达;CD8＋T淋巴细胞表面CD81,在HCV感染组、HIV感染组和HCV/HIV合并感染组中都呈高表达.外周血CD4＋T和CD8＋T淋巴细胞表面CCR5,在HIV感染组高表达,而在HCV感染组和HCV/HIV合并感染组中低表达.结论 HCV/HIV合并感染中,影响外周血CD4＋及CD8＋T淋巴细胞表面CCR5表达的主要因素是HCV感染;而合并感染对CD4＋及CD8＋T淋巴细胞表面CD81的表达,其影响作用是不相同的.

Correlation of receptor CD81 and CCR5 with HCV/HIV co-infection

Objective To investigate the expression of CD81 and CCR5 on CD4+/CD8+ T lymphocytes in the peripheral blood of patients with HCV mono-infection, HIV mono-infection and HCV/HIV co-infection as well as the correlation between them.Methods We measured the expression of CD81 and CCR5 on CD4+/CD8+ T lymphocytes in peripheral blood samples from 21 patients with HCV mono-infection, 14 patients with HIV mono-infection, 28 patients with HCV/HIV co-infection and 30 healthy volunteers by FACS.Results The expression of CD81 on CD4+ T lymphocytes in HCV mono-infection was not different from healthy control, but decreased significantly in HIV mono-infection and HCV/HIV co-infection. The expression of CD81 on CD8+ T lymphocytes increased significantly in HCV mono-infection, HIV mono-infection and HCV/HIV co-infection. The expression of CCR5 on CD4 + and CD8+ T lymphocytes increased significantly in HIV mono-infection, but decreased significantly in both HCV mono-infection and HCV/HIV co-infection.Conclusion In HCV/HIV co-infection, HCV may have effect on the expression of CCR5 on CD4+ and CD8+ T lymphocytes, while, HCV/HIV co-infection may have different effect on the expression of CD81 on CD4+ and CD8+ T lymphocytes from peripheral blood.