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| 高效液相色谱法测定家兔血浆中的乌头碱浓度 | |
| 引用本文： | 郭丹,林静瑜,周春权,姚欣,倪峰. 高效液相色谱法测定家兔血浆中的乌头碱浓度[J]. 海峡药学, 2011, 23(12): 32-34 |
| 作者姓名： | 郭丹 林静瑜 周春权 姚欣 倪峰 |
| 作者单位： | 1. 福建省中医药研究院 福州 350003 2. 福建卫生职业技术学院 福州 350101 |
| 基金项目： | 国家自然科学基金资助项目,福建省教育厅项目 |
| 摘 要： | 目的建立家兔血浆中乌头碱浓度的高效液相色谱（HPLC）测定方法。方法采用Symmetry shield RP18（5μm,4.6×250mm）色谱柱,以乙腈-0.1%甲酸（68∶32）为流动相,流速为0.8mL.min-1,检测波长为235nm。结果乌头碱在0.05～8μg.mL-1范围内成良好的线性关系（r2=0.999）。样品的回收率在95%～108%,日内和日间RSD均小于5%。结论本方法准确、灵敏、简便,适用于家兔血浆中乌头碱含量的测定分析。 |
| 关 键 词： | 血浆 乌头碱 高效液相色谱法 |
Determination of aconitine in rabbit plasma by HPLC |
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| GUO Dan , LIN Jing-yu , ZHOU Chun-quan , YAO Xin , NI Feng. Determination of aconitine in rabbit plasma by HPLC[J]. Strait Pharmaceutical Journal, 2011, 23(12): 32-34 | |
| Authors: | GUO Dan LIN Jing-yu ZHOU Chun-quan YAO Xin NI Feng |
| Affiliation: | 1.Fujian institiute of traditional Chinese medicine,Fuzhou,350003 China;2.Fujian Vocational college of health,Fuzhou.350101,China) |
| Abstract: | OBJECTIVE To establish a HPLC method for determination of aconitine in rabbit plasma.METHODS The samples were analyzed on the column of Symmetry shield RP18(5μm,4.6×250mm).The mobile phase consisted of acetonnitrile-0.1% formic acid(68∶32) at the rate of 0.8mL·min-1.The detective wavelength was at 235nm.RESULTS Good linearity was obtained in the range of 0.05～8μg·mL-1(r2=0.999).The recovery rate was 95～108%,both the inter-day and intra day RSD were less than 5%.CONCLUSION The method was accurate,sensitive,simple,and suitable for the deternination of aconitine in rabbit plasma. |
| Keywords: | plasma aconitine HPLC |
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