糖尿病大鼠膀胱细胞凋亡的改变

目的：研究糖尿病大鼠膀胱细胞凋亡情况及其意义.方法：实验分为正常对照组和糖尿病组.用10周龄SD大鼠腹腔内注射1％链脲佐菌素（STZ）建立糖尿病大鼠的动物模型.12周后取两组大鼠膀胱组织标本,原位细胞检测法检测细胞凋亡;免疫荧光方法检测CD146、PDGF-Rβ、NG-2表达;Western Blotting检测CD146、PDGF-Rβ表达.结果：与正常对照组比较,糖尿病组大鼠血糖升高,体质量减轻,膀胱湿重增加;细胞凋亡主要发生在糖尿病大鼠膀胱的尿路上皮细胞,而正常对照组未见明显尿路上皮细胞凋亡;两组大鼠膀胱组织中未见明显NG-2表达,CD146阳性表达细胞位于膀胱黏膜下层的血管壁及膀胱逼尿肌肌膜,PDGF-Rβ阳性表达细胞位于膀胱逼尿肌之间血管壁细胞及黏膜下血管壁;Western Blotting 检测结果显示,两组大鼠膀胱壁组织CD146、PDGF-Rβ的蛋白表达水平比较,差异无统计学意义（P＞0.05）.结论：糖尿病大鼠膀胱上皮细胞早期发即生凋亡改变,而周细胞早期凋亡不明显;上皮细胞的凋亡可能是糖尿病膀胱的治疗靶标之一.

Altered cell apoptosis in urinary bladder of diabetic rats

Objective： To investigate the cell apoptosis and its clinical significance in urinary bladder of diabetic rats. Methods： All rats were assigned to the normal control group and diabetic cystopathy group. Diabetic cystopathy models were established in 10-week SD rats by intraperitoneal injection of 1% streptozotoein. At week 12, the bladder tissues were harvested and the cell apoptosis in the tissue was detected with in-situ assays. The expression of CD146, PDGF-R~ and NG-2 was detected by immunofluorescence assay and expression of CD146 AND PDGF-RI3 by Western Blotting. Results： Compared with normal control group, diabetic cystopathy group showed high blood glucose, lowered body weights and increased wet-weight of the urinary bladder. Apoptosis of urothelial cells was found in diabetic cystopathy group, but not in NC group. Expression of NG-2 was negative in both groups. In addition, CD146-expressing cells were detected on vascular walls and the detrusor membrane in the cystic submucosa. By contrast, PDGF-R 3positive expression was found in the cells lining vascular walls between beth detrusors as well as in submocosal vascular walls. No signi~eant difference （ P 〉 O. 05） was revealed in the expression of CD146 and PDGF-R3 between both groups, as detected by Western blotting. Conclusion： Apoptosis of urothelial cells, but not pericytes, is involved in early-stage diabetic cystopathy in rats. Urothelial apoptosis may become one of the targets for treatment of diabetic cvstoDathv.