金黄地鼠胰岛素抵抗和糖尿病动物模型的建立

目的观察血管抑素基因联合氟尿嘧啶腹腔化疗对抗腹腔荷瘤裸小鼠的抗恶病质效应。方法RT-PCR克隆血管抑素基因,建立重组腺病毒载体。建立人结肠癌LoVo细胞腹腔种植的模型,给予血管抑素基因联合氟尿嘧啶腹腔化疗。观察荷瘤裸小鼠的体重和摄食量的改变及血清TNF-?和IL-1水平的变化。结果血管抑素基因联合氟尿嘧啶腹腔化疗组裸小鼠的体重、摄食量、精神和活动情况明显好于对照组;治疗组的血清TNF-?和IL-1水平和肿瘤微血管密度(MVD)明显低于其他各组。结论血管抑素的基因治疗与传统的腹腔化疗联合应用,可明显的改善荷瘤裸小鼠的恶病质症状,增强其抗恶病质的效应.

Establishment of an Insulin-Resistant and Type2 Diabetic Model in Golden Hamster

Objective To establish an experimental animal model with insulin resistance and/or type 2 diabetes mellitus.Methods High energy fructose diet was given to mesocricetus to produce insulin resistance and hyperlipidemic models.Diabetes was induced by a single intraperitoneal injection of streptozotocin(30 mg/kg)on these insulin resistant animals.Normal animals were used as control.Serum glucose,lipid profiles and insulin levels were measured to verify the diabetic and hyperlipidemic models.The liver,pancreas and aortic arch were examined to distinguish the histological difference among the animals of three groups.Results The mesocricetus fed with high energy fructose diet for 6 weeks developed insulin resistance,obesity and dislipidemia,80% of which became type 2 diabetic after single intraperitoneal injection of streptozotocin.Conclusion Insulin resistant and diabetic mesocricetus models can be produced successfully with high energy fructose diet and single intraperitoneal injection of streptozotocin.