IHC法与FISH法检测乳腺癌HER-2基因结果比较

目的比较免疫组织化学法(IHC)法和荧光原位杂交(FISH)法检测女性乳腺癌HER-2基因结果的可靠性。方法采用FISH双色法检测原发性浸润性乳腺癌中的HER-2基因及IHC检测HER-2蛋白表达情况,两者进行对比分析。结果在259例乳腺癌患者中,FISH检测阳性125例,IHC检测阳性239例,2者比较有显著性差异。将FISH结果和IHC结果进行分组比较,IHC为()者,FISH阳性率为76.19%;IHC为()者,FISH阳性率为52.25%。表明FISH和IHC有显著相关性。17号染色体非整倍体是造成IHC过度表达但FISH检测为阴性的主要原因。结论 IHC检测受实验室因素和主观因素影响,17号染色体非整倍体也是造成IHC过表达但基因不扩增的主要原因,所以确定HER-2扩增情况用FISH法检测更准确,能对患者的预后情况和治疗方案的选择提供更科学的依据。

Comparison of IHC method and FISH method in detection of breast cancer HER-2 gene

Objective It is to compare the reliability of results in exploration of female breast cancer HER-2 gene by immunohistochemical(IHC) method and fluorescence in situ hybridization(FISH) method.Methods The expression of HER-2 gene in primary invasive breast carcinoma was detected by FISH two-color assay,and the expression of HER-2 protein was detected by IHC method,then the results was compared and analyzed.Results In 259 patients with breast cancer,there were 125 cases with FISH of positive,239 cases with IHC of positive;there was significant difference between them.By group comparison,the FISH positive rate was 76.19% while IHC for(3+),and the FISH positive rate was 52.25% while IHC for(2+).There was significant positive correlation between FISH and IHC.The 17 chromosome aneuploidy was the main cause of IHC excessive expression but FISH detection for negative.Conclusion IHC detection is influenced by the factors of laboratory and subjective effects.The aneuploidy of chromosome 17 is the main cause of excessive expression of IHC but gene doesn't amplification.So the identification of HER-2 amplification by FISH assay is more accurate,the patient prognosis and treatment options to provide more scientific basis.