p53基因在涎腺多形性腺瘤发生中的基因序列突变

目的 检测腺多形性腺瘤ｐ５３基因第７、８外显子的碱基突变，探讨该瘤的发生及生物学特征。方法 从手术标本中选择涎腺多形性腺瘤１４例。采用聚合酶链反应－单链构象多态性分析技术及ＤＮＡ核酸序列测定方法对涎腺多形性腺瘤７例第７外显子异常者。３例第８外显子阳性者。５例第７外显子ＳＳＣＰ（其中３例第８外显子ＳＳＣＰ也呈阳性）的肿瘤进行核酸序列测定。结果 发现ＳＳＣＰ阳性的肿瘤相应的外显子碱基序列已发生变化。第

Tumour suppressor p53 gene sequence mutations in pleomorphic adenoma of salivary gland

OBJECTIVE: To detect p53 exon 7 and 8 mutation and to study genesis and biological characteristics of pleomorphic adenoma of salivary galand. METHODS: Polymerase chain reaction- single-strand conformational polymorphism (PCR-SSCP) and DNA sequencing analysis were used. RESULTS: PCR-SSCP analysis showed that in 11 patients with positive SSCP, 7 showed abnormal exon 7 and 3 abnormal exon 8. Comparison of SSCP results with DNA sequencing showed that patients all patients with p53 exon 7 and 8 mutations were SSCP positive. Exon 7 mutation pattern was point mutation(T-->C). Exon 8 mutation pattern included base substitution (point mutation) (C-->A) and frame-shiftmutation (base insertion and base loss). CONCLUSIONS: Polycodon and poly-pattern mutations appeared in p53 exon 7 and 8. Pleomorphic adenoma of salivary galand was similar to malignant tumor in p53 gene mutation. Poly-codon mutation could occur in the same case.