癌基因c-erbB-2特异性核酶对胃癌细胞恶性表型的逆转

目的 探讨ｃ－ｅｒｂＢ－２特异性核酶对胃癌细胞恶性表型的影响。方法 根据计算机设计的人癌基因ｃ－ｅｒｂＢ－２特异性核酶ＲＺ１,合成ＲＺ１基因并构建其真核表达载体ｐＤＯＲ－ＲＺ１,在Ｌｉｐｏｆｅｃｔ ＡＭＩＮＥ＾ＴＭ的介导下转染胃癌细胞ＳＧＣ－７９０１。流式细胞仪分析转染的胃癌ｃ－ｅｒｂＢ－２产物Ｐ１８５的表达。

Reversion of the malignant phenotype of gastric cancer by c-erbB-2 specific ribozyme

Objective To probe the effect of c erbB 2 specific ribozyme to the malignant phenotype of gastric cancer. Methods The eucaryotic expresion vector of c erbB 2 specific ribozyme RZ1 was designed by computer and named pDOR RZ1.The transfection of the gastric cancer cell line SGC 7901 was mediated by Lipofect AMINE TM .Flow cytometry was used to analyse the expression of the c erbB 2 product P185. In vivo study of tumorogenecity of the transfected cells was performed in nude mice. Results pDOR RZ1 was successfully transfected into the gastric cancer cell line SGC 7901 and then the single clones were selected by G418 and named SGC/RZ1. Flow cytometry analysis showed that the expression of P185 was suppressed by 62.7%. The growth rate of SGC/RZ1 was inhibited by 55%. The tumor forming time in SGC/RZ1 in nude mice was delayed remarkably and the tumor size was also much smaller than that in the control group, indicating the inhibition of the tumorogenecity of SGC/RZ1 in nude mice. Conclusion c erbB 2 specific ribozyme is very efficient in reversing the malignant phenotype of gastric cancer. This might provide a new approach for gene therapy of gastric cancer.