Evaluation of genetic polymorphisms in MMP2, MMP9 and MMP20 in Brazilian children with dental fluorosis |
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| Affiliation: | 1. Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China;2. Department of Neurology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China;3. Department of Neurosurgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China;4. The George Institute for Global Health, Royal Prince Alfred Hospital and University of Sydney, Sydney, NSW 2050, Australia |
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| Abstract: | ![]()
Recent studies suggested that genetics contribute to differences in dental fluorosis (DF) susceptibility among individuals having the same environmental exposure. This study evaluated if MMP2, MMP9 and MMP20 are expressed during enamel development and assessed the association between polymorphisms in these genes with DF. Mice susceptible and resistant to DF were used to evaluate if MMPs were candidate genes for DF. The animals received fluoride and their enamels were used for immunohistochemistry. Additionally, 481 subjects from a city with fluoridation of public water supplies were recruited. Genotyping was performed using real time PCR. Allele/genotype frequencies were compared between groups. MMP2, MMP9 and MMP20 immunostaining was detected in both animal groups. DF was observed in 22.4% of the subjects. A borderline association was observed in MMP2 (rs243865), MMP9 (rs17576) and in MMP20 (rs1784418) (p = 0.06, p = 0.08 and p = 0.06 respectively). Briefly, MMPs were expressed during enamel maturation and genetic polymorphisms were not associated with DF. |
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| Keywords: | Fluorosis Dental Immunohistochemistry Genetic susceptibility Matrix metalloproteinases Polymorphisms SNPs |
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